Drosophila nerve cord culture: A tool for studying neural development

Y. E. Wang; R. Chandler; P. Lau; A. J. Bieber

doi:10.1016/S0165-0270(98)00107-1

Drosophila nerve cord culture: A tool for studying neural development

Y. E. Wang, R. Chandler, P. Lau, A. J. Bieber

Neurosurgery

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The culture of explanted neural tissues has been a useful tool for the study of cellular and molecular neurobiology in vertebrates. We have developed a technique for the culture of explanted ventral nerve cords from Drosophila embryos. We have examined the morphology and dynamic behaviour of the growth cones that extend from these nerve cords, and the effect of calcium deprivation on the bundling of axons that are regenerated from the explanted tissue. This technique offers a unique opportunity to combine in vitro techniques for neuronal cell culture with the powerful techniques of genetic analysis that are available with Drosophila.

Original language	English (US)
Pages (from-to)	21-26
Number of pages	6
Journal	Journal of Neuroscience Methods
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/S0165-0270(98)00107-1
State	Published - Nov 1 1998

Keywords

Axon bundling
Calcium
Drosophila
Growth cone
Microtubule
Neurite outgrowth
Neuronal tissue culture

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0165-0270(98)00107-1

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title = "Drosophila nerve cord culture: A tool for studying neural development",

abstract = "The culture of explanted neural tissues has been a useful tool for the study of cellular and molecular neurobiology in vertebrates. We have developed a technique for the culture of explanted ventral nerve cords from Drosophila embryos. We have examined the morphology and dynamic behaviour of the growth cones that extend from these nerve cords, and the effect of calcium deprivation on the bundling of axons that are regenerated from the explanted tissue. This technique offers a unique opportunity to combine in vitro techniques for neuronal cell culture with the powerful techniques of genetic analysis that are available with Drosophila.",

keywords = "Axon bundling, Calcium, Drosophila, Growth cone, Microtubule, Neurite outgrowth, Neuronal tissue culture",

author = "Wang, {Y. E.} and R. Chandler and P. Lau and Bieber, {A. J.}",

note = "Funding Information: This work was supported by a grant from the Burroughs Wellcome Fund of the Appalachian College Association to R.C., by grants from National Science Foundation (IBN-9410068) and National Institute of Health (NS 09074) to David Bentley, and by grants from the Showalter Foundation and the National Science Foundation (IBN-9120981) to A.J.B. We also thank Mr and Mrs Eugene Applebaum for their generous financial support. ",

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T1 - Drosophila nerve cord culture

T2 - A tool for studying neural development

AU - Wang, Y. E.

AU - Chandler, R.

AU - Lau, P.

AU - Bieber, A. J.

N1 - Funding Information: This work was supported by a grant from the Burroughs Wellcome Fund of the Appalachian College Association to R.C., by grants from National Science Foundation (IBN-9410068) and National Institute of Health (NS 09074) to David Bentley, and by grants from the Showalter Foundation and the National Science Foundation (IBN-9120981) to A.J.B. We also thank Mr and Mrs Eugene Applebaum for their generous financial support.

PY - 1998/11/1

Y1 - 1998/11/1

N2 - The culture of explanted neural tissues has been a useful tool for the study of cellular and molecular neurobiology in vertebrates. We have developed a technique for the culture of explanted ventral nerve cords from Drosophila embryos. We have examined the morphology and dynamic behaviour of the growth cones that extend from these nerve cords, and the effect of calcium deprivation on the bundling of axons that are regenerated from the explanted tissue. This technique offers a unique opportunity to combine in vitro techniques for neuronal cell culture with the powerful techniques of genetic analysis that are available with Drosophila.

AB - The culture of explanted neural tissues has been a useful tool for the study of cellular and molecular neurobiology in vertebrates. We have developed a technique for the culture of explanted ventral nerve cords from Drosophila embryos. We have examined the morphology and dynamic behaviour of the growth cones that extend from these nerve cords, and the effect of calcium deprivation on the bundling of axons that are regenerated from the explanted tissue. This technique offers a unique opportunity to combine in vitro techniques for neuronal cell culture with the powerful techniques of genetic analysis that are available with Drosophila.

KW - Axon bundling

KW - Calcium

KW - Drosophila

KW - Growth cone

KW - Microtubule

KW - Neurite outgrowth

KW - Neuronal tissue culture

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JO - Journal of Neuroscience Methods

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IS - 1

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Drosophila nerve cord culture: A tool for studying neural development

Abstract

Keywords

ASJC Scopus subject areas

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