Donor-specific hypo-responsiveness occurs in simultaneous liver-kidney transplant recipients after the first year

Timucin Taner, Michael P. Gustafson, Michael J. Hansen, Walter D. Park, Svetlana Bornschlegl, Allan B. Dietz, Mark D. Stegall

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Kidney allografts of patients who undergo simultaneous liver-kidney transplantation incur less immune-mediated injury, and retain better function compared to other kidney allografts. To characterize the host alloimmune responses in 28 of these patients, we measured the donor-specific alloresponsiveness and phenotypes of peripheral blood cells after the first year. These values were then compared to those of 61 similarly immunosuppressed recipients of a solitary kidney or 31 recipients of liver allografts. Four multicolor, non-overlapping flow cytometry protocols were used to assess the immunophenotypes. Mixed cell cultures with donor or third party cells were used to measure cell proliferation and interferon gamma production. Despite a significant overlap, simultaneous liver-kidney transplant recipients had a lower overall frequency of circulating CD8+, activated CD4+ and effector memory T cells, compared to solitary kidney transplant recipients. Simultaneous liver-kidney transplant recipient T cells had a significantly lower proliferative response to the donor cells compared to solitary kidney recipients (11.9 vs. 42.9%), although their response to third party cells was unaltered. The frequency of interferon gamma producing alloreactive T cells in simultaneous liver-kidney transplant recipients was significantly lower than that of solitary kidney transplant recipients. Flow cytometric analysis of the mixed cultures demonstrated that both alloreactive CD4+ and CD8+ compartments of the simultaneous liver-kidney transplant recipient circulating blood cells were smaller. Thus, the phenotypic and functional characteristics of the circulating blood cells of the simultaneous liver-kidney transplant recipients resembled those of solitary liver transplant recipients, and appear to be associated with donor-specific hypo-alloresponsiveness.

Original languageEnglish (US)
Pages (from-to)1465-1474
Number of pages10
JournalKidney international
Issue number6
StatePublished - Jun 2018


  • alloimmunity
  • immunoregulation
  • kidney transplantation
  • rejection
  • simultaneous liver-kidney transplantation

ASJC Scopus subject areas

  • Nephrology


Dive into the research topics of 'Donor-specific hypo-responsiveness occurs in simultaneous liver-kidney transplant recipients after the first year'. Together they form a unique fingerprint.

Cite this