DNA Modifications Enabling Proximity Biotinylation

Brandon Wilbanks, Keenan Pearson, Shane R. Byrne, Laura B. Bickart, Peter C. Dedon, L. James Maher

Research output: Contribution to journalArticlepeer-review


Advances in peroxidase and biotin ligase-mediated signal amplification have enabled high-resolution subcellular mapping of endogenous RNA localization and protein-protein interactions. Application of these technologies has been limited to RNA and proteins because of the reactive groups required for biotinylation in each context. Here we report several novel methods for proximity biotinylation of exogenous oligodeoxyribonucleotides by application of well-established and convenient enzymatic tools. We describe approaches using simple and efficient conjugation chemistries to modify deoxyribonucleotides with “antennae” that react with phenoxy radicals or biotinoyl-5′-adenylate. In addition, we report chemical details of a previously undescribed adduct between tryptophan and a phenoxy radical group. These developments have potential application in the selection of exogenous nucleic acids capable of unaided entry into living cells.

Original languageEnglish (US)
JournalBioconjugate Chemistry
StateAccepted/In press - 2022

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry


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