Differentiation of human neural stem cells into retinal cells

X. Dong; J. S. Pulido; T. Qu; K. Sugaya

doi:10.1097/00001756-200301200-00026

Differentiation of human neural stem cells into retinal cells

X. Dong, J. S. Pulido, T. Qu, K. Sugaya

Ophthalmology

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

We have previously reported that transplanted human neural stem cells (HNSCs) display extensive migration and positional incorporation into the aged rat brain, which is associated with an improvement in cognitive function. In the current study, to investigate whether HNSCs are capable of differentiating into retinal cells, we treated HNSCs with human transforming growth factor-β3 (TGF-β3) under a serum-free differentiation condition. After 5 days of differentiation in vitro we detected opsin-immuno-positive cells in the culture treated with TGF-β3. We also transplanted TGF-β3-treated HNSCs into the rat vitreous cavity. The donor cells migrated and differentiated into opsin-positive cells in the host retinal cell layer. Here we show for the first time that TGF-β3-treated HNSCs differentiate into retinal cells. Neuro. (c) 2003 Lippincott Williams & Wilkins.

Original language	English (US)
Pages (from-to)	143-146
Number of pages	4
Journal	NeuroReport
Volume	14
Issue number	1
DOIs	https://doi.org/10.1097/00001756-200301200-00026
State	Published - Jan 20 2003

Keywords

Neural stem cell
Photoreceptor
Retina
Retinal degeneration
Transforming growth factor
Transplantation

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-200301200-00026

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AU - Pulido, J. S.

AU - Qu, T.

AU - Sugaya, K.

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Differentiation of human neural stem cells into retinal cells

Abstract

Keywords

ASJC Scopus subject areas

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