TY - CHAP
T1 - Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment
AU - Ruiz-Garcia, Henry
AU - Zarco, Natanael
AU - Watanabe, Fumihiro
AU - De Araujo Farias, Virginea
AU - Suarez-Meade, Paola
AU - Guerrero-Cazares, Hugo
AU - Imitola, Jaime
AU - Quinones-Hinojosa, Alfredo
AU - Trifiletti, Daniel
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Glioblastoma (GBM) is the most common and dismal primary brain tumor. Unfortunately, despite multidisciplinary treatment, most patients will perish approximately 15 months after diagnosis. For this reason, there is an urgent need to improve our understanding of GBM tumor biology and develop novel therapies that can achieve better clinical outcomes. In this setting, three-dimensional tumor models have risen as more appropriate preclinical tools when compared to traditional cell cultures, given that two-dimensional (2D) cultures have failed to accurately recapitulate tumor biology and translate preclinical findings into patient benefits. Three-dimensional cultures using neurospheres, organoids, and organotypic better resemble original tumor genetic and epigenetic profiles, maintaining tumor microenvironment characteristics and mimicking cell–cell and cell–matrix interactions. This chapter summarizes our methods to generate well-characterized glioblastoma neurospheres, organoids, and organotypics.
AB - Glioblastoma (GBM) is the most common and dismal primary brain tumor. Unfortunately, despite multidisciplinary treatment, most patients will perish approximately 15 months after diagnosis. For this reason, there is an urgent need to improve our understanding of GBM tumor biology and develop novel therapies that can achieve better clinical outcomes. In this setting, three-dimensional tumor models have risen as more appropriate preclinical tools when compared to traditional cell cultures, given that two-dimensional (2D) cultures have failed to accurately recapitulate tumor biology and translate preclinical findings into patient benefits. Three-dimensional cultures using neurospheres, organoids, and organotypic better resemble original tumor genetic and epigenetic profiles, maintaining tumor microenvironment characteristics and mimicking cell–cell and cell–matrix interactions. This chapter summarizes our methods to generate well-characterized glioblastoma neurospheres, organoids, and organotypics.
KW - Glioblastoma
KW - Neurosphere
KW - Organoids
KW - Organotypics
KW - Stem cell
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U2 - 10.1007/978-1-0716-2703-7_9
DO - 10.1007/978-1-0716-2703-7_9
M3 - Chapter
C2 - 36161412
AN - SCOPUS:85138611809
T3 - Methods in Molecular Biology
SP - 117
EP - 127
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -