TY - JOUR
T1 - Determination of skeletal muscle triglyceride synthesis using a single muscle biopsy
AU - Guo, Zeng Kui
AU - Jensen, Michael D.
N1 - Funding Information:
From the Endocrine Research Unit, Mayo Foundation, Rochester, MN. Submitted November 27, 2001; accepted March 15, 2002. Supported by Grant No. DK 40484 from the National Institutes of Health and Grant No. DK50456 from the Minnesota Obesity Center. Address reprint requests to ZengKui Guo, PhD, 5-194 Joseph, Mayo Foundation, Rochester, MN 55905. Copyright 2002, Elsevier Science (USA). All rights reserved. 0026-0495/02/5109-0017$35.00/0 doi:10.1053/meta.2002.34711
PY - 2002/9
Y1 - 2002/9
N2 - A novel stable isotopic technique for the determination of triglyceride synthesis in skeletal muscle by using a single muscle biopsy has been developed and evaluated in rats. In previous studies using 13C-tracers, muscle triglyceride synthesis is usually determined using at least 2 biopsies, the first of which serves as the baseline sample for the measurement of natural 13C abundance. In the present studies, the baseline biopsy has been eliminated by making the use of the isotopic information of a nontraced fatty acid in the muscle triglyceride pool. This is based on the fact that the source and, hence, the natural 13C abundance of fatty acids in the same triglyceride pool is similar. To demonstrate and validate the method, a series of rat studies have been conducted to have established that (1) the natural 13C abundance of 4 major fatty acids in the muscle triglyceride pool is similar; (2) there are no 13C-label exchanges between fatty acids in the lipid pool; and (3) the incorporation of 13C-palmitate into muscle triglycerides determined using this technique favorably compared with that determined by the traditional method. This approach makes stable isotope studies possible in which more than 1 muscle biopsy is difficult or impossible. Therefore, it has the potential to facilitate investigation of triglyceride metabolism in the skeletal muscle.
AB - A novel stable isotopic technique for the determination of triglyceride synthesis in skeletal muscle by using a single muscle biopsy has been developed and evaluated in rats. In previous studies using 13C-tracers, muscle triglyceride synthesis is usually determined using at least 2 biopsies, the first of which serves as the baseline sample for the measurement of natural 13C abundance. In the present studies, the baseline biopsy has been eliminated by making the use of the isotopic information of a nontraced fatty acid in the muscle triglyceride pool. This is based on the fact that the source and, hence, the natural 13C abundance of fatty acids in the same triglyceride pool is similar. To demonstrate and validate the method, a series of rat studies have been conducted to have established that (1) the natural 13C abundance of 4 major fatty acids in the muscle triglyceride pool is similar; (2) there are no 13C-label exchanges between fatty acids in the lipid pool; and (3) the incorporation of 13C-palmitate into muscle triglycerides determined using this technique favorably compared with that determined by the traditional method. This approach makes stable isotope studies possible in which more than 1 muscle biopsy is difficult or impossible. Therefore, it has the potential to facilitate investigation of triglyceride metabolism in the skeletal muscle.
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U2 - 10.1053/meta.2002.34711
DO - 10.1053/meta.2002.34711
M3 - Article
C2 - 12200767
AN - SCOPUS:0036740377
SN - 0026-0495
VL - 51
SP - 1198
EP - 1205
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 9
ER -