Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera

S. Yokota, W. P. Lafuse, J. F. McCormick, C. S. David

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Ia specificities 22 and 23 were initially identified by using conventional alloantisera and were mapped to the I-E subregion of k and d haplotypes on the basis of their reactivity with selected recombinants. Recently we found that Ia 22 and Ia 23 are hybrid determinants on the basis of their expression on selected F1 cells, but absence from both parental cells. Initial attempts to detect hybrid Ia antigens by immunizing parents with F1 cells were unsucessful. By utilizing lipopolysaccharide (LPS)-stimulated F1 spleen lymphoblasts as immunogens, 1 of the parents as recipient and the other parent cells for absorption of antisera, specific anti-Ia.22 and 23 antibodies were produced. The specificity of these parent anti-F1 sera was confirmed by cytotoxic and immunoprecipitation analyses.

Original languageEnglish (US)
Pages (from-to)371-374
Number of pages4
JournalJournal of Immunology
Issue number1
StatePublished - 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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