@inbook{72f907cf18d24affbb5b732dd881bc1d,
title = "Detection of Circulating RNA Using Nanopore Sequencing",
abstract = "RNA sequencing using nanopore sequencing is a powerful method for transcriptome analysis. The approach is appropriate for comprehensive profiling of the wide range of long noncoding RNAs. Use of nanopore-based sequencing can provide information on novel transcripts, sequence polymorphisms, and splicing variants, and thus has advantages over other gene expression profiling methods such as microarrays. Circulating extracellular long noncoding RNAs are of particular interest because of their potential use as biomarkers. Here, we describe a protocol for cDNA-PCR sequencing of circulating RNA for biomarker discovery in whole blood samples using commercially available kits and nanopore sequencing.",
keywords = "Biofluids, Biomarker discovery, Long noncoding RNA, Nanopore sequencing, RNA sequencing",
author = "Jennifer Lindemann and Yan, {Irene K.} and Tushar Patel",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1581-2_19",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "273--284",
booktitle = "Methods in Molecular Biology",
}