Detection of Circulating RNA Using Nanopore Sequencing

Jennifer Lindemann, Irene K. Yan, Tushar Patel

Research output: Chapter in Book/Report/Conference proceedingChapter


RNA sequencing using nanopore sequencing is a powerful method for transcriptome analysis. The approach is appropriate for comprehensive profiling of the wide range of long noncoding RNAs. Use of nanopore-based sequencing can provide information on novel transcripts, sequence polymorphisms, and splicing variants, and thus has advantages over other gene expression profiling methods such as microarrays. Circulating extracellular long noncoding RNAs are of particular interest because of their potential use as biomarkers. Here, we describe a protocol for cDNA-PCR sequencing of circulating RNA for biomarker discovery in whole blood samples using commercially available kits and nanopore sequencing.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Biofluids
  • Biomarker discovery
  • Long noncoding RNA
  • Nanopore sequencing
  • RNA sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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