Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.

R. Cattaneo; H. Will; G. Darai; E. Pfaff; H. Schaller

doi:10.1002/j.1460-2075.1983.tb01455.x

Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.

R. Cattaneo, H. Will, G. Darai, E. Pfaff, H. Schaller

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Plasmids containing hepatitis B virus (HBV) DNA and a 232-bp SV40 DNA fragment encoding the origin of replication were constructed. When introduced by transfection into COS cells, these plasmids directed the synthesis of hepatitis B surface antigen. S1 mapping of the mRNAs covering the S gene showed that transcriptional initiation was promoted by the interaction of HBV sequences with an SV40 promoter element: transcription started on HBV DNA but had several properties of SV40 late transcription. The detection of a promoter element in an SV40 origin fragment commonly used in the COS system is important for the interpretation of data deriving from expression studies in COS cells.

Original language	English (US)
Pages (from-to)	511-514
Number of pages	4
Journal	The EMBO journal
Volume	2
Issue number	4
DOIs	https://doi.org/10.1002/j.1460-2075.1983.tb01455.x
State	Published - 1983

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1002/j.1460-2075.1983.tb01455.x

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title = "Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.",

abstract = "Plasmids containing hepatitis B virus (HBV) DNA and a 232-bp SV40 DNA fragment encoding the origin of replication were constructed. When introduced by transfection into COS cells, these plasmids directed the synthesis of hepatitis B surface antigen. S1 mapping of the mRNAs covering the S gene showed that transcriptional initiation was promoted by the interaction of HBV sequences with an SV40 promoter element: transcription started on HBV DNA but had several properties of SV40 late transcription. The detection of a promoter element in an SV40 origin fragment commonly used in the COS system is important for the interpretation of data deriving from expression studies in COS cells.",

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AU - Cattaneo, R.

AU - Will, H.

AU - Darai, G.

AU - Pfaff, E.

AU - Schaller, H.

PY - 1983

Y1 - 1983

N2 - Plasmids containing hepatitis B virus (HBV) DNA and a 232-bp SV40 DNA fragment encoding the origin of replication were constructed. When introduced by transfection into COS cells, these plasmids directed the synthesis of hepatitis B surface antigen. S1 mapping of the mRNAs covering the S gene showed that transcriptional initiation was promoted by the interaction of HBV sequences with an SV40 promoter element: transcription started on HBV DNA but had several properties of SV40 late transcription. The detection of a promoter element in an SV40 origin fragment commonly used in the COS system is important for the interpretation of data deriving from expression studies in COS cells.

AB - Plasmids containing hepatitis B virus (HBV) DNA and a 232-bp SV40 DNA fragment encoding the origin of replication were constructed. When introduced by transfection into COS cells, these plasmids directed the synthesis of hepatitis B surface antigen. S1 mapping of the mRNAs covering the S gene showed that transcriptional initiation was promoted by the interaction of HBV sequences with an SV40 promoter element: transcription started on HBV DNA but had several properties of SV40 late transcription. The detection of a promoter element in an SV40 origin fragment commonly used in the COS system is important for the interpretation of data deriving from expression studies in COS cells.

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Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.

Abstract

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