Denervation-induced changes in myosin heavy chain expression in the rat diaphragm muscle

Paige C. Geiger; Jeffrey P. Bailey; Wen Zhi Zhan; Carlos B. Mantilla; Gary C. Sieck

doi:10.1152/japplphysiol.00862.2002

Denervation-induced changes in myosin heavy chain expression in the rat diaphragm muscle

Paige C. Geiger, Jeffrey P. Bailey, Wen Zhi Zhan, Carlos B. Mantilla, Gary C. Sieck

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Unilateral denervation (Dnv) of the rat diaphragm muscle (Dia_m) markedly alters expression of myosin heavy chain (MHC) isoforms. After 2 wk of Dia_m Dnv, MHC content per half-sarcomere decreases in fibers expressing MHC_2X and MHC_2B. We hypothesized that changes in MHC protein expression parallel changes in MHC mRNA expression. Relative MHC isoform mRNA levels were determined by Northern analysis after 1, 3, 7, and 14 days of Dnv of the rat Dia_m. MHC protein expression was determined by SDS-PAGE. Changes in MHC isoform protein and mRNA expression were not concurrent. Expression of MHC_Slow and MHC_2X mRNA isoforms decreased dramatically by 3 days of Dnv, whereas that of MHC_2A and MHC_2B did not change. Expression of all MHC protein isoforms decreased by 3 days of Dnv. We observed a differential effect of rat Dia_m Dnv on MHC isoform protein and mRNA expression. The time course of the changes in MHC isoform mRNA and protein expression suggests a predominant effect of altered protein turnover rates on MHC protein expression instead of altered transcription after Dnv.

Original language	English (US)
Pages (from-to)	611-619
Number of pages	9
Journal	Journal of applied physiology
Volume	95
Issue number	2
DOIs	https://doi.org/10.1152/japplphysiol.00862.2002
State	Published - Aug 1 2003

Keywords

Electrophoresis
Innervation patterns
Myosin heavy chain gene regulation
Neuromuscular control

ASJC Scopus subject areas

Physiology
Physiology (medical)

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10.1152/japplphysiol.00862.2002

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abstract = "Unilateral denervation (Dnv) of the rat diaphragm muscle (Diam) markedly alters expression of myosin heavy chain (MHC) isoforms. After 2 wk of Diam Dnv, MHC content per half-sarcomere decreases in fibers expressing MHC2X and MHC2B. We hypothesized that changes in MHC protein expression parallel changes in MHC mRNA expression. Relative MHC isoform mRNA levels were determined by Northern analysis after 1, 3, 7, and 14 days of Dnv of the rat Diam. MHC protein expression was determined by SDS-PAGE. Changes in MHC isoform protein and mRNA expression were not concurrent. Expression of MHCSlow and MHC2X mRNA isoforms decreased dramatically by 3 days of Dnv, whereas that of MHC2A and MHC2B did not change. Expression of all MHC protein isoforms decreased by 3 days of Dnv. We observed a differential effect of rat Diam Dnv on MHC isoform protein and mRNA expression. The time course of the changes in MHC isoform mRNA and protein expression suggests a predominant effect of altered protein turnover rates on MHC protein expression instead of altered transcription after Dnv.",

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AU - Geiger, Paige C.

AU - Bailey, Jeffrey P.

AU - Zhan, Wen Zhi

AU - Mantilla, Carlos B.

AU - Sieck, Gary C.

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Y1 - 2003/8/1

N2 - Unilateral denervation (Dnv) of the rat diaphragm muscle (Diam) markedly alters expression of myosin heavy chain (MHC) isoforms. After 2 wk of Diam Dnv, MHC content per half-sarcomere decreases in fibers expressing MHC2X and MHC2B. We hypothesized that changes in MHC protein expression parallel changes in MHC mRNA expression. Relative MHC isoform mRNA levels were determined by Northern analysis after 1, 3, 7, and 14 days of Dnv of the rat Diam. MHC protein expression was determined by SDS-PAGE. Changes in MHC isoform protein and mRNA expression were not concurrent. Expression of MHCSlow and MHC2X mRNA isoforms decreased dramatically by 3 days of Dnv, whereas that of MHC2A and MHC2B did not change. Expression of all MHC protein isoforms decreased by 3 days of Dnv. We observed a differential effect of rat Diam Dnv on MHC isoform protein and mRNA expression. The time course of the changes in MHC isoform mRNA and protein expression suggests a predominant effect of altered protein turnover rates on MHC protein expression instead of altered transcription after Dnv.

AB - Unilateral denervation (Dnv) of the rat diaphragm muscle (Diam) markedly alters expression of myosin heavy chain (MHC) isoforms. After 2 wk of Diam Dnv, MHC content per half-sarcomere decreases in fibers expressing MHC2X and MHC2B. We hypothesized that changes in MHC protein expression parallel changes in MHC mRNA expression. Relative MHC isoform mRNA levels were determined by Northern analysis after 1, 3, 7, and 14 days of Dnv of the rat Diam. MHC protein expression was determined by SDS-PAGE. Changes in MHC isoform protein and mRNA expression were not concurrent. Expression of MHCSlow and MHC2X mRNA isoforms decreased dramatically by 3 days of Dnv, whereas that of MHC2A and MHC2B did not change. Expression of all MHC protein isoforms decreased by 3 days of Dnv. We observed a differential effect of rat Diam Dnv on MHC isoform protein and mRNA expression. The time course of the changes in MHC isoform mRNA and protein expression suggests a predominant effect of altered protein turnover rates on MHC protein expression instead of altered transcription after Dnv.

KW - Electrophoresis

KW - Innervation patterns

KW - Myosin heavy chain gene regulation

KW - Neuromuscular control

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Denervation-induced changes in myosin heavy chain expression in the rat diaphragm muscle

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Keywords

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