Dendritic cells strongly boost the antitumor activity of adoptively transferred T cells in vivo

Yanyan Lou, Gang Wang, Gregory Lizée, Grace J. Kim, Steven E. Finkelstein, Chiguang Feng, Nicholas P. Restifo, Patrick Hwu

Research output: Contribution to journalArticlepeer-review

100 Scopus citations


Dendritic cells (DCs) have been well characterized for their ability to initiate cell-mediated immune responses by stimulating naive T cells. However, the use of DCs to stimulate antigen-activated T cells in vivo has not been investigated. In this study, we determined whether DC vaccination could improve the efficacy of activated, adoptively transferred T cells to induce an enhanced antitumor immune response. Mice bearing B16 melanoma tumors expressing the gp100 tumor antigen were treated with cultured, activated T cells transgenic for a T-cell receptor specifically recognizing gp100, with or without concurrent peptide-pulsed DC vaccination. In this model, antigen-specific DC vaccination induced cytokine production, enhanced proliferation, and increased tumor infiltration of adoptively transferred T cells. Furthermore, the combination of DC vaccination and adoptive T-cell transfer led to a more robust antitumor response than the use of each treatment individually. Collectively, these findings illuminate a new potential application for DCs in the in vivo stimulation of adoptively transferred T cells and may be a useful approach for the immunotherapy of cancer.

Original languageEnglish (US)
Pages (from-to)6783-6790
Number of pages8
JournalCancer research
Issue number18
StatePublished - Sep 15 2004

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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