TY - JOUR
T1 - Cytokine indices in Alzheimer's temporal cortex
T2 - no changes in mature IL-1β or IL-1RA but increases in the associated acute phase proteins IL-6, α2-macroglobulin and C-reactive protein
AU - Wood, Julie A.
AU - Wood, Paul L.
AU - Ryan, Randall
AU - Graff-Radford, Neill R.
AU - Pilapil, Carmencita
AU - Robitaille, Yves
AU - Quirion, Remi
N1 - Funding Information:
Acknowledgements. This work was supported by the Mayo Foundation for Medical Research and Grant NIA AG 08031-0251.
PY - 1993/12/3
Y1 - 1993/12/3
N2 - Recent immunocytochemical data have demonstrated increases in interleukin-1β (IL-1β), interleukin-6 (IL-6) and the IL-6-inducible acute phase protein, α2-macroglobulin (α2-M), in Alzheimer's disease (AD) brains. We investigated the levels of these proteins quantitatively using ELISA procedures and determined if increases in IL-1β were compensated for by a parallel increase in the endogenous interleukin-1 receptor antagonist (IL-1RA). Comparing control vs. Alzheimer's temporal cortex, we examined mature IL-1β, IL-1RA, IL-6, α2-M and C-reactive protein (CRP). The specificities of the ELISA procedures were verified by serial dilutions of the samples; by chromatofocusing, and by Sephadex G-150 gel filtration. There were no differences in the levels of mature IL-1β or IL-1RA in AD and control brains. However, IL-6 levels were detectable in 14 of the 16 Alzheimer samples but only 2 of the 14 control samples. There were also significant increases seen in α2-M and CRP levels in the Alzheimer's group compared to controls. These data support previous studies demonstrating a possible up-regulation of neuroimmune function in Alzheimer's cortex; however, we cannot determine, at this time, if this immune reaction is initiated by IL-1β.
AB - Recent immunocytochemical data have demonstrated increases in interleukin-1β (IL-1β), interleukin-6 (IL-6) and the IL-6-inducible acute phase protein, α2-macroglobulin (α2-M), in Alzheimer's disease (AD) brains. We investigated the levels of these proteins quantitatively using ELISA procedures and determined if increases in IL-1β were compensated for by a parallel increase in the endogenous interleukin-1 receptor antagonist (IL-1RA). Comparing control vs. Alzheimer's temporal cortex, we examined mature IL-1β, IL-1RA, IL-6, α2-M and C-reactive protein (CRP). The specificities of the ELISA procedures were verified by serial dilutions of the samples; by chromatofocusing, and by Sephadex G-150 gel filtration. There were no differences in the levels of mature IL-1β or IL-1RA in AD and control brains. However, IL-6 levels were detectable in 14 of the 16 Alzheimer samples but only 2 of the 14 control samples. There were also significant increases seen in α2-M and CRP levels in the Alzheimer's group compared to controls. These data support previous studies demonstrating a possible up-regulation of neuroimmune function in Alzheimer's cortex; however, we cannot determine, at this time, if this immune reaction is initiated by IL-1β.
KW - Acute phase protein
KW - Alzheimer's disease
KW - C-reactive protein
KW - Interleukin-1 receptor antagonist
KW - Interleukin-1β
KW - Interleukin-6
KW - α2-Macroglobulin
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U2 - 10.1016/0006-8993(93)91327-O
DO - 10.1016/0006-8993(93)91327-O
M3 - Article
C2 - 7509248
AN - SCOPUS:0027453380
SN - 0006-8993
VL - 629
SP - 245
EP - 252
JO - Brain Research
JF - Brain Research
IS - 2
ER -