Abstract
Cyclic ADP-ribose (cADPR) was shown to induce calcium release from the endoplasmic reticulum via ryanodine-sensitive pathways. In smooth muscle, two pathways for calcium release from the sarcoplasmic reticulum (SR) have been previously demonstrated: D-myo-inositol 1,4,5-trisphosphate-gated and ryanodine-gated. However, evidence for cADPR as a regulator for SR Ca2+ release in smooth muscle is lacking. We used permeabilized porcine coronary artery smooth muscle cells to directly examine the stimulation of SR Ca2+ release by cADPR. The results provide direct evidence that cADPR stimulates SR Ca2+ release and that this response is not inhibited by heparin, by depletion of the caffeine-sensitive Ca2+ pool, or by blockade of ryanodine receptors. These results indicate a novel mechanism for Ca2+ release from the SR of vascular smooth muscle.
Original language | English (US) |
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Pages (from-to) | H801-H806 |
Journal | American Journal of Physiology - Heart and Circulatory Physiology |
Volume | 270 |
Issue number | 2 39-2 |
DOIs | |
State | Published - 1996 |
Keywords
- caffeine
- dissociated cells
- fluorescence imaging
- heparin
- ryanodine
- β- escin
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
- Physiology (medical)