TY - JOUR
T1 - CXCR4+ and FLK-1+ identify circulating cells associated with improved cardiac function in patients following myocardial infarction
AU - Suresh, Rahul
AU - Chiriac, Anca
AU - Goel, Kashish
AU - Villarraga, Hector R.
AU - Lopez-Jimenez, Francisco
AU - Thomas, Randal J.
AU - Terzic, Andre
AU - Nelson, Timothy J.
AU - Perez-Terzic, Carmen
N1 - Funding Information:
Funding This work was supported in part by the Todd and Karen Wanek Program for Hypoplastic Left Heart Syndrome, Marriott Regenerative Medicine Award, and Mayo Clinic. It was also supported by CTSA Grant Number UL1 TR000135 and TL1 TR000137 from the National Center for Advancing Translational Science (NCATS).
PY - 2013/10
Y1 - 2013/10
N2 - The biomarkers CXCR4/FLK-1 select cardiac progenitors from a stem cell pool in experimental models. However, the translational value of these cells in human ischemic heart disease is unknown. Here, flow-cytometry identified CD45-/CXCR4+/FLK-1+ cells in 30 individuals without ischemic heart disease and 33 first-time acute myocardial infarction (AMI) patients. AMI patients had higher CD45-/CXCR4 +/FLK-1+ cell-load at 48-h and 3- and 6-months post-AMI (p = 0.003,0.04,0.04, respectively) than controls. Cardiovascular risk factors and left ventricular (LV) ejection fraction were not associated with cell-load. 2D-speckle-tracking strain echocardiography assessment of LV systolic function showed improvement in longitudinal strain and dyssynchrony during follow-up associated with longitudinal increases in and higher 48-h post-AMI CD45 -/CXCR4+/FLK-1+ cell-load (r = -0.525, p = 0.025; r = -0.457, p = 0.029, respectively). In conclusion, CD45 -/CXCR4+/FLK-1+ cells are present in adult human circulation, increased in AMI and associated with improved LV systolic function. Thus, CD45-/CXCR4+/FLK-1+ cells may provide a diagnostic tool to follow cardiac regenerative capacity and potentially serve as a prognostic marker in AMI.
AB - The biomarkers CXCR4/FLK-1 select cardiac progenitors from a stem cell pool in experimental models. However, the translational value of these cells in human ischemic heart disease is unknown. Here, flow-cytometry identified CD45-/CXCR4+/FLK-1+ cells in 30 individuals without ischemic heart disease and 33 first-time acute myocardial infarction (AMI) patients. AMI patients had higher CD45-/CXCR4 +/FLK-1+ cell-load at 48-h and 3- and 6-months post-AMI (p = 0.003,0.04,0.04, respectively) than controls. Cardiovascular risk factors and left ventricular (LV) ejection fraction were not associated with cell-load. 2D-speckle-tracking strain echocardiography assessment of LV systolic function showed improvement in longitudinal strain and dyssynchrony during follow-up associated with longitudinal increases in and higher 48-h post-AMI CD45 -/CXCR4+/FLK-1+ cell-load (r = -0.525, p = 0.025; r = -0.457, p = 0.029, respectively). In conclusion, CD45 -/CXCR4+/FLK-1+ cells are present in adult human circulation, increased in AMI and associated with improved LV systolic function. Thus, CD45-/CXCR4+/FLK-1+ cells may provide a diagnostic tool to follow cardiac regenerative capacity and potentially serve as a prognostic marker in AMI.
KW - CXCR4
KW - Cardiac progenitor cells
KW - FLK-1
KW - Longitudinal strain
KW - Myocardial infarction
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U2 - 10.1007/s12265-013-9502-z
DO - 10.1007/s12265-013-9502-z
M3 - Article
C2 - 23934537
AN - SCOPUS:84885674187
SN - 1937-5387
VL - 6
SP - 787
EP - 797
JO - Journal of cardiovascular translational research
JF - Journal of cardiovascular translational research
IS - 5
ER -