Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton; Kevin Loutherback; David W. Inglis; James C. Sturm; Robert H. Austin; Stephen Y. Chou

Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton, Kevin Loutherback, David W. Inglis, James C. Sturm, Robert H. Austin, Stephen Y. Chou

Laboratory Medicine and Pathology

Research output: Contribution to conference › Paper › peer-review

Abstract

We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

Original language	English (US)
Pages	1965-1967
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Cell lysis
Cell separation
Genomics
Sorting

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Crossing streamlines to lyse cells and simultaneously separate genomic contents. / Morton, Keith J.; Loutherback, Kevin; Inglis, David W. et al.
2008. 1965-1967 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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abstract = "We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.",

keywords = "Cell lysis, Cell separation, Genomics, Sorting",

author = "Morton, {Keith J.} and Kevin Loutherback and Inglis, {David W.} and Sturm, {James C.} and Austin, {Robert H.} and Chou, {Stephen Y.}",

note = "Copyright: Copyright 2014 Elsevier B.V., All rights reserved.; 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

year = "2008",

language = "English (US)",

pages = "1965--1967",

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T1 - Crossing streamlines to lyse cells and simultaneously separate genomic contents

AU - Morton, Keith J.

AU - Loutherback, Kevin

AU - Inglis, David W.

AU - Sturm, James C.

AU - Austin, Robert H.

AU - Chou, Stephen Y.

PY - 2008

Y1 - 2008

N2 - We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

AB - We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

KW - Cell lysis

KW - Cell separation

KW - Genomics

KW - Sorting

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M3 - Paper

AN - SCOPUS:84902440707

SP - 1965

EP - 1967

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Crossing streamlines to lyse cells and simultaneously separate genomic contents

Abstract

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Keywords

ASJC Scopus subject areas

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