Correlation of DNA ploidy with c-erbB-2 expression in preinvasive and invasive breast tumors

D. W. Visscher, F. H. Sarkar, J. D. Crissman

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Detection of c-erbB-2 oncogene product expression by monoclonal antibody staining (avidin-biotin technique) in formalin-fixed, paraffin-embedded atypical hyperplasias (AH, n=20), intraductal carcinomas (IDCA, n=27) and invasive carcinomas (INVCA, n=48) was compared to ploidy determinations obtained by flow cytometry (INVCA) or image analysis (AH, IDCA). Cytoplasmic membrane staining was present in 11/48 (23%) INVCA and 8/27 (30%) IDCA but none of the AH. Tumors with abnormal DNA content expressed c-erbB-2 more frequently: INVCA, 2/19 (11%) diploid range versus 9/29 (31%) aneuploid; IDCA, 1/7 (14%) diploid range versus 7/20 (35%) aneuploid. Poorly differentiated (nuclear grade) IDCA or INVCA were also more frequently stained (14/35, 40%) than were well or moderately differentiated cases (5/40, 12.5%). Oncogene product expression and DNA content derangements may be related biologic parameters in breast neoplasia, and both are highly associated with cytologic nuclear abnormalities.

Original languageEnglish (US)
Pages (from-to)418-424
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Issue number6
StatePublished - 1991

ASJC Scopus subject areas

  • Anatomy
  • Histology


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