Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

Pouria Fattahi; Ali Rahimian; Michael Q. Slama; Kihak Gwon; Alan M. Gonzalez-Suarez; Jadon Wolf; Harihara Baskaran; Caden D. Duffy; Gulnaz Stybayeva; Quinn P. Peterson; Alexander Revzin

doi:10.1038/s41598-021-85786-2

Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

Pouria Fattahi, Ali Rahimian, Michael Q. Slama, Kihak Gwon, Alan M. Gonzalez-Suarez, Jadon Wolf, Harihara Baskaran, Caden D. Duffy, Gulnaz Stybayeva, Quinn P. Peterson, Alexander Revzin

Physiology & Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Cellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.

Original language	English (US)
Article number	7177
Journal	Scientific reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-85786-2
State	Published - Dec 2021

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Fattahi, P., Rahimian, A., Slama, M. Q., Gwon, K., Gonzalez-Suarez, A. M., Wolf, J., Baskaran, H., Duffy, C. D., Stybayeva, G., Peterson, Q. P., & Revzin, A. (2021). Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor. Scientific reports, 11(1), Article 7177. https://doi.org/10.1038/s41598-021-85786-2

Fattahi, P, Rahimian, A, Slama, MQ, Gwon, K, Gonzalez-Suarez, AM, Wolf, J, Baskaran, H, Duffy, CD, Stybayeva, G, Peterson, QP & Revzin, A 2021, 'Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor', Scientific reports, vol. 11, no. 1, 7177. https://doi.org/10.1038/s41598-021-85786-2

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abstract = "Cellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.",

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AU - Gwon, Kihak

AU - Gonzalez-Suarez, Alan M.

AU - Wolf, Jadon

AU - Baskaran, Harihara

AU - Duffy, Caden D.

AU - Stybayeva, Gulnaz

AU - Peterson, Quinn P.

AU - Revzin, Alexander

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Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

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