Abstract
In total, 50 Escherichia coli bloodstream isolates from the clinical laboratory and 12 E. coli isolates referred for pulsed-field gel electrophoresis (PFGE) were sequenced, assessed for clonality using core genome multilocus sequence typing (cgMLST), and evaluated for genomic susceptibility predictions using ARESdb. Results of sequence typing using whole-genome sequencing (WGS)-based MLST and sequence type (ST)-specific PCR were identical. Overall categorical agreement between genotypic (ARESdb) and phenotypic susceptibility testing for 62 isolates and 11 antimicrobial agents was 91%. Among the referred isolates, high major error rates were found for ceftazidime, cefepime, and piperacillin-tazobactam.
Original language | English (US) |
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Article number | e01139-21 |
Journal | Antimicrobial Agents and Chemotherapy |
Volume | 65 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2021 |
Keywords
- Antibiotic susceptibility prediction
- Escherichia coli
- Molecular typing
- Whole-genome sequencing
ASJC Scopus subject areas
- Pharmacology
- Pharmacology (medical)
- Infectious Diseases