TY - GEN
T1 - Continuous Electrocardiogram Monitoring in Zebrafish with Prolonged Mild Anesthesia
AU - Le, Tai
AU - Zhang, Jimmy
AU - Xia, Xing
AU - Xu, Xiaolei
AU - Clark, Isaac
AU - Schmiess-Heine, Lauren
AU - Nguyen, Anh H.
AU - Lau, Michael P.H.
AU - Cao, Hung
N1 - Publisher Copyright:
© 2020 IEEE.
PY - 2020/7
Y1 - 2020/7
N2 - The zebrafish model has been demonstrated as an ideal vertebrate model system for a diverse range of biological studies. Along with conventional approaches, monitoring and analysis of zebrafish electrocardiogram (ECG) have been utilized for cardio-physiological screening and elucidation. ECG monitoring has been carried out with fish treated with anesthetic drugs, rendering the short period of time in recording the signals (<5 min). In this work, a prolonged sedation system for continuous ECG monitoring of multiple zebrafish was proposed and developed. We built a circulation system to provide prolonged mild anesthesia which allows more consistent and intrinsic ECG measurement. The use of prolonged anesthesia helped reduce the concentration of the anesthetic drug (MS222 or Tricaine) from 200 mg/L to 100 mg/L and even lower; thus, maintaining the integrity of intrinsic ECG. Moreover, heartrate variation during recording was investigated, showing minute changes (±3.2 beats per minute - BPM). The development of this prolonged ECG monitoring system would open the possibility of long-term monitoring for studies such as drug screening and forward genetic screening.
AB - The zebrafish model has been demonstrated as an ideal vertebrate model system for a diverse range of biological studies. Along with conventional approaches, monitoring and analysis of zebrafish electrocardiogram (ECG) have been utilized for cardio-physiological screening and elucidation. ECG monitoring has been carried out with fish treated with anesthetic drugs, rendering the short period of time in recording the signals (<5 min). In this work, a prolonged sedation system for continuous ECG monitoring of multiple zebrafish was proposed and developed. We built a circulation system to provide prolonged mild anesthesia which allows more consistent and intrinsic ECG measurement. The use of prolonged anesthesia helped reduce the concentration of the anesthetic drug (MS222 or Tricaine) from 200 mg/L to 100 mg/L and even lower; thus, maintaining the integrity of intrinsic ECG. Moreover, heartrate variation during recording was investigated, showing minute changes (±3.2 beats per minute - BPM). The development of this prolonged ECG monitoring system would open the possibility of long-term monitoring for studies such as drug screening and forward genetic screening.
KW - Cardiac Disease
KW - Drug Screening
KW - ECG
KW - Prolonged Anesthesia
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85091034843&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85091034843&partnerID=8YFLogxK
U2 - 10.1109/EMBC44109.2020.9175576
DO - 10.1109/EMBC44109.2020.9175576
M3 - Conference contribution
AN - SCOPUS:85091034843
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 2610
EP - 2613
BT - 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society, EMBC 2020
Y2 - 20 July 2020 through 24 July 2020
ER -