TY - JOUR
T1 - Confocal Microscopy in Ophthalmology
AU - Erie, Jay C.
AU - McLaren, Jay W.
AU - Patel, Sanjay V.
N1 - Funding Information:
This study was supported in part by Research to Prevent Blindness Inc, New York, New York (A Restricted Departmental Grant, and SVP as Olga Keith Wiess Special Scholar), and Mayo Foundation, Mayo Clinic, Rochester, Minnesota. The authors indicate no financial conflict of interest. All authors were involved in design and conduct of study; collection of data; management, analysis, and interpretation of data; and preparation, review, and approval of manuscript.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/11
Y1 - 2009/11
N2 - Purpose: To describe the principles, capabilities, and applications of confocal microscopy in vivo in ophthalmology. Design: Perspective, literature review, and commentary. Methods: Review and synthesis of selected recent literature, with interpretation and perspective. Results: Confocal microscopy imaging has led to a better understanding of the cellular microstructure in the normal, postsurgical, and diseased cornea by enabling quantitative analysis of the cellular response in the human cornea in vivo. At present, the major role of confocal microscopy is in research of corneal surgery and disease. Clinical applications are limited to facilitating the diagnosis of Acanthamoeba and deep fungal keratitis, measuring residual bed thickness after laser in situ keratomileusis, and measuring endothelial cell density in high-light-scattering situations. Conclusions: In addition to providing qualitative data, confocal microscopy is valuable for quantitative analysis of the cornea and will enable the investigation of pharmacologic and surgical modifications of corneal wound healing, nerve regeneration, and cellular responses. Prospective, quantitative analyses require individual calibration of confocal microscopes for lateral and axial dimensions of images, for image depth, and for light intensity.
AB - Purpose: To describe the principles, capabilities, and applications of confocal microscopy in vivo in ophthalmology. Design: Perspective, literature review, and commentary. Methods: Review and synthesis of selected recent literature, with interpretation and perspective. Results: Confocal microscopy imaging has led to a better understanding of the cellular microstructure in the normal, postsurgical, and diseased cornea by enabling quantitative analysis of the cellular response in the human cornea in vivo. At present, the major role of confocal microscopy is in research of corneal surgery and disease. Clinical applications are limited to facilitating the diagnosis of Acanthamoeba and deep fungal keratitis, measuring residual bed thickness after laser in situ keratomileusis, and measuring endothelial cell density in high-light-scattering situations. Conclusions: In addition to providing qualitative data, confocal microscopy is valuable for quantitative analysis of the cornea and will enable the investigation of pharmacologic and surgical modifications of corneal wound healing, nerve regeneration, and cellular responses. Prospective, quantitative analyses require individual calibration of confocal microscopes for lateral and axial dimensions of images, for image depth, and for light intensity.
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U2 - 10.1016/j.ajo.2009.06.022
DO - 10.1016/j.ajo.2009.06.022
M3 - Article
C2 - 19674730
AN - SCOPUS:70350566528
SN - 0002-9394
VL - 148
SP - 639
EP - 646
JO - American journal of ophthalmology
JF - American journal of ophthalmology
IS - 5
ER -