Concomitant activation and antigen uptake via human Dectin-1 results in potent antigen-specific CD8+ T cell responses

Ling Ni, Ingrid Gayet, Sandra Zurawski, Dorothee Duluc, Anne Laure Flamar, Xiao Hua Li, Amy O'Bar, Sandra Clayton, Anna Karolina Palucka, Gerard Zurawski, Jacques Banchereau, Sang Kon Oh

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


Dectin-1, a C-type lectin recognizing fungal and mycobacterial pathogens, can deliver intracellular signals that activate dendritic cells (DCs), resulting in initiation of immune responses and expansion of Th17 CD4+ T cell responses. In this paper, we studied the roles of human Dectin-1 (hDectin-1) expressed on DCs in the induction and activation of Ag-specific CD8+ T cell responses. We first generated an agonistic anti-hDectin-1 mAb, which recognizes the hDectin-1 Glu143-Ile162 region. It bound to in vitro monocyte-derived DCs and to in vivo CD1c+CD1a+ dermal DCs but not to epidermal Langerhans cells. Anti-hDectin-1-mediated DC activation resulted in upregulation of costimulatory molecules and secretion of multiple cytokines and chemokines in a Syk-dependent manner. DCs activated with the anti-hDectin-1 mAb could significantly enhance both neo and foreign Ag-specific CD8+ T cell responses by promoting both the expansion of CD8+ T cells and their functional activities. We further demonstrated that delivering Ags to DCs via hDectin-1 using anti-hDectin-1-Ag conjugates resulted in potent Ag-specific CD8+ T cell responses. Thus, hDectin-1 expressed on DCs can contribute to the induction and activation of cellular immunity against intracellular pathogens, such as mycobacteria, that are recognized by DCs via Dectin-1. Vaccines based on delivering Ags to DCs with an agonistic anti-hDectin-1 mAb could elicit CD8+ T cell-mediated immunity. Copyright

Original languageEnglish (US)
Pages (from-to)3504-3513
Number of pages10
JournalJournal of Immunology
Issue number6
StatePublished - Sep 15 2010

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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