@article{996eaab191054392940de8e28f31b36a,
title = "Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity",
abstract = "Estrogen Receptor-β (ERβ) has been implicated in many cancers. In prostate and breast cancer its function is controversial, but genetic studies implicate a role in cancer progression. Much of the confusion around ERβ stems from antibodies that are inadequately validated, yet have become standard tools for deciphering its role. Using an ERβ-inducible cell system we assessed commonly utilized ERβ antibodies and show that one of the most commonly used antibodies, NCL-ER-BETA, is non-specific for ERβ. Other antibodies have limited ERβ specificity or are only specific in one experimental modality. ERβ is commonly studied in MCF-7 (breast) and LNCaP (prostate) cancer cell lines, but we found no ERβ expression in either, using validated antibodies and independent mass spectrometry-based approaches. Our findings question conclusions made about ERβ using the NCL-ER-BETA antibody, or LNCaP and MCF-7 cell lines. We describe robust reagents, which detect ERβ across multiple experimental approaches and in clinical samples.",
keywords = "Antibody, Breast, Cancer, Estrogen receptor beta, Prostate",
author = "Nelson, {Adam W.} and Groen, {Arnoud J.} and Miller, {Jodi L.} and Warren, {Anne Y.} and Holmes, {Kelly A.} and Tarulli, {Gerard A.} and Tilley, {Wayne D.} and Katzenellenbogen, {Benita S.} and Hawse, {John R.} and Gnanapragasam, {Vincent J.} and Carroll, {Jason S.}",
note = "Funding Information: AWN is an Honorary Research Fellow of the Royal College of Surgeons of England and acknowledges their support. The authors are grateful to Dr. Rasmus Siersbaek for providing MCF-7 cells, Ms. Marie Pickering for her assistance with IHC, and the staff of the Cambridge Institute's core facilities, particularly Biorepository, Proteomics, Histopathology and Research Core Instrumentation Facility. The authors acknowledge the Breast Cancer Research Foundation , Cancer Research UK , ERC Consolidator award (grant number 646876 ), Cambridge Biomedical Research Campus and Cambridge Cancer Centre , which fund the tissue bank and the Urology Biorepository. Funding Information: AWN is supported by The Medical Research Council ( MR/L00156X/1 ) and The Urology Foundation Scholarship ( RESCH1302 ). WDT is supported by the National Health and Medical Research Council of Australia (ID 1008349 and 1084416 ), Cancer Australia/National Breast Cancer Foundation (ID 1043497 ), National Breast Cancer Foundation Pilot Study ( PS-15-041 ) and a Prostate Cancer Research Program Transformative Impact Award from the US Department of Defense ( W81XWH-13-2-0093 ). BSK receives a grant from the Breast Cancer Research Foundation . JSC is funded by Cancer Research UK and an ERC Consolidator award . Publisher Copyright: {\textcopyright} 2016 The Authors",
year = "2017",
month = jan,
day = "15",
doi = "10.1016/j.mce.2016.11.016",
language = "English (US)",
volume = "440",
pages = "138--150",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
}