CNP production in the kidney and effects of protein intake restriction in nephrotic syndrome

Alessandro Cataliotti, Mauro Giordano, Emanuela De Pascale, Gelsomina Giordano, Pietro Castellino, Michihisa Jougasaki, Lisa C. Costello, Guido Boerrigter, Toshihiro Tsuruda, Paola Belluardo, Shang Chiun Lee, Brenda Huntley, Sharon Sandberg, Lorenzo S. Malatino, John C. Burnett

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


C-type natriuretic peptide (CNP) possesses well-established cardiovascular properties. Although present in the mammalian kidney, CNP production in human kidney and its modulation in human renal disease remain less defined. We investigated the presence of CNP in normal human kidney and in patients with nephrotic syndrome (NS). We also addressed whether or not a low-protein diet (LPD) alters plasma CNP and urinary CNP excretion in NS. In situ hybridization studies demonstrated CNP mRNA expression in tubular cells and glomeruli of normal human kidneys. CNP immunoreactivity was positive in proximal, distal, and medullary collecting duct tubular cells in both controls and patients with NS. The ratios of plasma CNP and urinary CNP to creatinine were significantly higher in patients with NS compared with controls. Urinary CNP, but not plasma CNP, was significantly lowered in patients with NS after an LPD. Similarly, the ratios of urinary protein to creatinine and urinary albumin to creatinine, but not urinary guanosine 3′,5′-cyclic monophosphate to creatinine, decreased significantly with an LPD. These data confirm and extend previous reports and demonstrate for the first time the presence of CNP in human kidney with NS. We also report increased plasma CNP concentration and urinary CNP excretion in NS patients and a significant reduction of CNP excretion with an LPD. Our findings demonstrate that CNP metabolism is altered in patients with NS and support the hypothesis that activation of renal CNP can be partially offset by an LPD. These results underscore that the beneficial effect of an LPD on protein excretion is paralleled by a substantial reduction in intrarenal CNP release.

Original languageEnglish (US)
Pages (from-to)F464-F472
JournalAmerican Journal of Physiology - Renal Physiology
Issue number3 52-3
StatePublished - Sep 2002


  • Immunohistochemistry
  • In situ hybridization
  • Low protein diet
  • Urinary C-type natriuretic peptide

ASJC Scopus subject areas

  • Physiology
  • Urology


Dive into the research topics of 'CNP production in the kidney and effects of protein intake restriction in nephrotic syndrome'. Together they form a unique fingerprint.

Cite this