Cloning of Human Alloreactive T Cells

Jorg Goronzy, Cornelia Weyand, C. Garrison Fathman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


This chapter discusses cloning of human alloreactive T cells. Human alloreactive T cell clones have proved invaluable for the immunogenetic analysis of MHC-encoded determinants. Polyclonal cell populations, used initially to define allostimulatory specificities on HLA class I and class II molecules, do not reflect single T cell interaction sites on the target molecules. Alloreactive human T cell clones will undoubtedly be of use in HLA typing, especially in identifying HLA determinants for organ transplantation. Analysis of the effector and regulatory functions of such T cells as well as an understanding of the genetic factors controlling cellular interactions have benefited from analysis using clonal populations. When stimulated in vitro, T cells secrete soluble factors called “lymphokines,” and cloned T cells are ideal sources to study the induction and biochemical nature of these T cell hormones.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalMethods in enzymology
Issue numberC
StatePublished - Jan 1987

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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