TY - JOUR
T1 - Cloning of Human Alloreactive T Cells
AU - Goronzy, Jorg
AU - Weyand, Cornelia
AU - Garrison Fathman, C.
PY - 1987/1
Y1 - 1987/1
N2 - This chapter discusses cloning of human alloreactive T cells. Human alloreactive T cell clones have proved invaluable for the immunogenetic analysis of MHC-encoded determinants. Polyclonal cell populations, used initially to define allostimulatory specificities on HLA class I and class II molecules, do not reflect single T cell interaction sites on the target molecules. Alloreactive human T cell clones will undoubtedly be of use in HLA typing, especially in identifying HLA determinants for organ transplantation. Analysis of the effector and regulatory functions of such T cells as well as an understanding of the genetic factors controlling cellular interactions have benefited from analysis using clonal populations. When stimulated in vitro, T cells secrete soluble factors called “lymphokines,” and cloned T cells are ideal sources to study the induction and biochemical nature of these T cell hormones.
AB - This chapter discusses cloning of human alloreactive T cells. Human alloreactive T cell clones have proved invaluable for the immunogenetic analysis of MHC-encoded determinants. Polyclonal cell populations, used initially to define allostimulatory specificities on HLA class I and class II molecules, do not reflect single T cell interaction sites on the target molecules. Alloreactive human T cell clones will undoubtedly be of use in HLA typing, especially in identifying HLA determinants for organ transplantation. Analysis of the effector and regulatory functions of such T cells as well as an understanding of the genetic factors controlling cellular interactions have benefited from analysis using clonal populations. When stimulated in vitro, T cells secrete soluble factors called “lymphokines,” and cloned T cells are ideal sources to study the induction and biochemical nature of these T cell hormones.
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U2 - 10.1016/0076-6879(87)50091-X
DO - 10.1016/0076-6879(87)50091-X
M3 - Article
C2 - 3501522
AN - SCOPUS:0023462726
SN - 0076-6879
VL - 150
SP - 333
EP - 341
JO - Methods in enzymology
JF - Methods in enzymology
IS - C
ER -