TY - JOUR
T1 - Cloning of B cell lymphoma-associated antigens using modified phage-displayed expression cDNA library and immunized patient sera
AU - Cha, Soung Chul
AU - Kwak, Larry W.
AU - Ruffini, Pier Adelchi
AU - Qin, Hong
AU - Neelapu, Sattva
AU - Biragyn, Arya
N1 - Funding Information:
We thank Alison Woo for editorial assistance. This work was supported by a grant from the Ladies Leukemia League.
PY - 2006/5/30
Y1 - 2006/5/30
N2 - Active immunization of follicular lymphoma patients with idiotypic vaccines elicits antigen-specific antibody responses, T-cell responses, and antitumor effects. We hypothesized that these vaccinated patients could generate tumor-specific immune responses, not only against idiotype, but also against other tumor-associated antigens (TAA) by a mechanism of epitope spreading. To identify potential antigens, a phage surface expressed cDNA library derived from primary tumor cells was screened with sera from idiotype-vaccinated patients. Consistent with our hypothesis, we identified two immunogenic peptides (FL-aa-7 and 18), unrelated to idiotype, which were recognized by postvaccine sera but not by prevaccine or normal human sera. These peptide sequences derived from the 5′-untranslated regions of the human GTPase, IMAP family member 7 gene (FL-aa-7) and an alternative reading frame of U1-snRNP 70 (FL-aa-18), respectively, suggesting that epitope spreading had occurred.
AB - Active immunization of follicular lymphoma patients with idiotypic vaccines elicits antigen-specific antibody responses, T-cell responses, and antitumor effects. We hypothesized that these vaccinated patients could generate tumor-specific immune responses, not only against idiotype, but also against other tumor-associated antigens (TAA) by a mechanism of epitope spreading. To identify potential antigens, a phage surface expressed cDNA library derived from primary tumor cells was screened with sera from idiotype-vaccinated patients. Consistent with our hypothesis, we identified two immunogenic peptides (FL-aa-7 and 18), unrelated to idiotype, which were recognized by postvaccine sera but not by prevaccine or normal human sera. These peptide sequences derived from the 5′-untranslated regions of the human GTPase, IMAP family member 7 gene (FL-aa-7) and an alternative reading frame of U1-snRNP 70 (FL-aa-18), respectively, suggesting that epitope spreading had occurred.
KW - B cell lymphoma
KW - Epitope spreading
KW - Phage display
KW - Tumor antigen discovery
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U2 - 10.1016/j.jim.2006.02.013
DO - 10.1016/j.jim.2006.02.013
M3 - Article
C2 - 16631194
AN - SCOPUS:33646910521
SN - 0022-1759
VL - 312
SP - 79
EP - 93
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -