Cloning and Recombinant Expression of a Cellulase

Research output: Chapter in Book/Report/Conference proceedingChapter


Cellulases are very important enzymes that break up cellulose. This reaction is used, for example, for biofuel production. A reduction in cellulase production cost, an improvement in cellulase performance, and an increase in sugar yields are all vital to reduce the processing costs of biorefineries. Improvements in specific cellulase activities for noncomplexed cellulase mixtures can be implemented through cellulase engineering based on rational design or directed evolution for each cellulase component enzyme, as well as on the reconstitution of cellulase components. This chapter explains the cloning of the cellulase gene from cellulolytic bacteria into Escherichia coli.

Original languageEnglish (US)
Title of host publicationNew and Future Developments in Microbial Biotechnology and Bioengineering
Subtitle of host publicationMicrobial Cellulase System Properties and Applications
PublisherElsevier Inc.
Number of pages8
ISBN (Electronic)9780444635150
ISBN (Print)9780444635075
StatePublished - Aug 2 2016


  • Biofuel
  • Cellulase
  • Cellulolytic bacteria
  • Cloning and expression
  • Lignocellulose

ASJC Scopus subject areas

  • Immunology and Microbiology(all)


Dive into the research topics of 'Cloning and Recombinant Expression of a Cellulase'. Together they form a unique fingerprint.

Cite this