Cigarette smoke induces immune responses to vimentin in both, arthritis-susceptible and -resistant humanized Mice

Mitali Bidkar, Robert Vassallo, David Luckey, Michele Smart, Kelly Mouapi, Veena Taneja

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12 Scopus citations


Rheumatoid arthritis (RA) is an autoimmune disease marked by chronic synovial inflammation and both, genetic and environmental factors are involved in its pathogenesis. Human leukocyte antigen (HLA) DRB1∗0401 is associated with susceptibility to develop RA, while cigarette smoke (CS) exposure promotes seropositive disease with increased severity in DRB1∗0401+ individuals. Smokers have higher levels of antibodies against citrullinated peptides. In this study, we determined whether the response to a known autoantigen, Vimentin (Vim) is shared epitope specific and how CS influences this response using transgenic-mice carrying RA-susceptible,∗0401, and -resistant, ∗0402, genes. Following relatively brief exposure to CS, peptidyl arginine deiminase (PAD) enzyme expression was increased in murine lungs. Cigarette smoking led to production of Interferon (IFN)-ã with reduced levels of Interleukin (IL)-10 by splenocytes of ∗0401 mice. In contrast, CS augmented Th2 cytokines along with T-regulatory cells in ∗0402 mice. An increase in levels of antibodies to native and citrullinated Vim was observed in naïve mice of both strains following CS exposure. Our data showed that both arthritis-susceptible and -resistant mice can generate cellular and humoral immunity to Vim; however CSinduced modulation of host immunity is dependent on the interaction with the host HLA genes.

Original languageEnglish (US)
Article numbere0162341
JournalPloS one
Issue number9
StatePublished - Sep 2016

ASJC Scopus subject areas

  • General


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