Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma

Erica A. Power, Jenelys Fernandez-Torres, Liang Zhang, Ruiyi Yaun, Fabrice Lucien, David J. Daniels

Research output: Contribution to journalArticlepeer-review


Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. We found that patient-derived DIPG tumors develop in the CAM and maintain the same genetic and epigenetic characteristics of native DIPG tumors. We monitored tumor response to pharmaco- and radiation therapy by 3-D ultrasound volumetric and vasculature analysis. In this study, we established and validated the CAM model as a potential intermediate xenograft model for DIPG and its use for testing novel treatment approaches that include pharmacotherapy or radiation.

Original languageEnglish (US)
Article numbere0263822
JournalPloS one
Issue number2
StatePublished - Feb 2022

ASJC Scopus subject areas

  • General


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