Characterization of the link protein from bovine epiphyseal plate

Peter C. Amadio; Benjamin V. Treadwell; Christine A. Towle; Henry J. Mankin

doi:10.1016/S0006-291X(80)80094-5

Characterization of the link protein from bovine epiphyseal plate

Peter C. Amadio, Benjamin V. Treadwell, Christine A. Towle, Henry J. Mankin

Research output: Contribution to journal › Article › peer-review

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Abstract

The link protein fraction of bovine epiphyseal plate cartilage was isolated using a method previously described by this laboratory. Amino acid analysis, polyacrylamide gel electrophoresis, and in vitro proteoglycan aggregation studies gave results similar to those previously reported for bovine nasal and articular links. Immunodiffusion studies of epiphyseal plate and articular links showed that the two tissues have immunologically indistinguishable link fractions. As in other bovine cartilages, two main link proteins were identified, but the lower molecular weight protein was more prominent in epiphyseal than in nasal or articular cartilage.

Original language	English (US)
Pages (from-to)	1695-1702
Number of pages	8
Journal	Topics in Catalysis
Volume	95
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(80)80094-5
State	Published - 1980

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Biophysics
Molecular Biology

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10.1016/S0006-291X(80)80094-5

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@article{acda5c5b06f64a6eb25cdb3fdf46aaeb,

title = "Characterization of the link protein from bovine epiphyseal plate",

abstract = "The link protein fraction of bovine epiphyseal plate cartilage was isolated using a method previously described by this laboratory. Amino acid analysis, polyacrylamide gel electrophoresis, and in vitro proteoglycan aggregation studies gave results similar to those previously reported for bovine nasal and articular links. Immunodiffusion studies of epiphyseal plate and articular links showed that the two tissues have immunologically indistinguishable link fractions. As in other bovine cartilages, two main link proteins were identified, but the lower molecular weight protein was more prominent in epiphyseal than in nasal or articular cartilage.",

author = "Amadio, {Peter C.} and Treadwell, {Benjamin V.} and Towle, {Christine A.} and Mankin, {Henry J.}",

note = "Funding Information: Acknowledgements. The work presented here was supported by United States Public Health Service Grant /~I 16265. Peter C. ~:~adio was the recipient of a fellowship from the New England Peabody Foundation. We are grateful to D. P. Mankin for his helpful suggestions.",

year = "1980",

doi = "10.1016/S0006-291X(80)80094-5",

language = "English (US)",

volume = "95",

pages = "1695--1702",

journal = "Topics in Catalysis",

number = "4",

}

TY - JOUR

T1 - Characterization of the link protein from bovine epiphyseal plate

AU - Amadio, Peter C.

AU - Treadwell, Benjamin V.

AU - Towle, Christine A.

AU - Mankin, Henry J.

N1 - Funding Information: Acknowledgements. The work presented here was supported by United States Public Health Service Grant /~I 16265. Peter C. ~:~adio was the recipient of a fellowship from the New England Peabody Foundation. We are grateful to D. P. Mankin for his helpful suggestions.

PY - 1980

Y1 - 1980

N2 - The link protein fraction of bovine epiphyseal plate cartilage was isolated using a method previously described by this laboratory. Amino acid analysis, polyacrylamide gel electrophoresis, and in vitro proteoglycan aggregation studies gave results similar to those previously reported for bovine nasal and articular links. Immunodiffusion studies of epiphyseal plate and articular links showed that the two tissues have immunologically indistinguishable link fractions. As in other bovine cartilages, two main link proteins were identified, but the lower molecular weight protein was more prominent in epiphyseal than in nasal or articular cartilage.

AB - The link protein fraction of bovine epiphyseal plate cartilage was isolated using a method previously described by this laboratory. Amino acid analysis, polyacrylamide gel electrophoresis, and in vitro proteoglycan aggregation studies gave results similar to those previously reported for bovine nasal and articular links. Immunodiffusion studies of epiphyseal plate and articular links showed that the two tissues have immunologically indistinguishable link fractions. As in other bovine cartilages, two main link proteins were identified, but the lower molecular weight protein was more prominent in epiphyseal than in nasal or articular cartilage.

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JO - Topics in Catalysis

JF - Topics in Catalysis

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ER -

Characterization of the link protein from bovine epiphyseal plate

Abstract

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