TY - JOUR
T1 - Characterization of monoclonal antibodies against rabbit CD44
T2 - Evidence of a role for CD44 in modulating synoviocyte metabolism
AU - Galea-Lauri, Joanna
AU - Wilkinson, J. Michael
AU - Evans, C. H.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993/10
Y1 - 1993/10
N2 - The CD44 adhesion receptor is a hyaluronan-binding cell surface glycoprotein whose major isoform is a 95 kDa molecule with a wide tissue distribution. We have characterized two MAbs, W4/86 and RPN3/24, by immunochemistry, flow cytometry and fluorescence staining and demonstrated that they recognize the rabbit CD44 molecule. The results show that CD44 in the rabbit has a similar molecular size and cell distribution to human CD44. These antibodies have demonstrated for the first time, the presence of CD44 on synoviocytes. Preincubation of the rabbit synovial cell line HIG-82 with W4/86 inhibited its synthesis of PGE2 in response to autocrine synovial cytokines. These results indicate that the CD44 molecule may help to modulate synoviocyte metabolism.
AB - The CD44 adhesion receptor is a hyaluronan-binding cell surface glycoprotein whose major isoform is a 95 kDa molecule with a wide tissue distribution. We have characterized two MAbs, W4/86 and RPN3/24, by immunochemistry, flow cytometry and fluorescence staining and demonstrated that they recognize the rabbit CD44 molecule. The results show that CD44 in the rabbit has a similar molecular size and cell distribution to human CD44. These antibodies have demonstrated for the first time, the presence of CD44 on synoviocytes. Preincubation of the rabbit synovial cell line HIG-82 with W4/86 inhibited its synthesis of PGE2 in response to autocrine synovial cytokines. These results indicate that the CD44 molecule may help to modulate synoviocyte metabolism.
UR - http://www.scopus.com/inward/record.url?scp=0027382807&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027382807&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(93)90099-W
DO - 10.1016/0161-5890(93)90099-W
M3 - Article
C2 - 7694087
AN - SCOPUS:0027382807
SN - 0161-5890
VL - 30
SP - 1383
EP - 1392
JO - Molecular Immunology
JF - Molecular Immunology
IS - 15
ER -