Characterization of human breast cancer by scanning acoustic microscopy

Di Chen, Eugene Malyarenko, Fedar Seviaryn, Ye Yuan, Mark Sherman, Sudeshna Bandyopadhyay, Gretchen Gierach, Christopher W. Greenway, Elena Maeva, Emil Strumban, Neb Duric, Roman Maev

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Objectives: The purpose of this study was to characterize human breast cancer tissues by the measurement of microacoustic properties. Methods: We investigated eight breast cancer patients using acoustic microscopy. For each patient, seven blocks of tumor tissue were collected from seven different positions around a tumor mass. Frozen sections (10 micrometer, μm) of human breast cancer tissues without staining and fixation were examined in a scanning acoustic microscope with focused transducers at 80 and 200 MHz. Hematoxylin and Eosin (H&E) stained sections from the same frozen breast cancer tissues were imaged by optical microscopy for comparison. Results: The results of acoustic imaging showed that acoustic attenuation and sound speed in cancer cell-rich tissue regions were significantly decreased compared with the surrounding tissue regions, where most components are normal cells/tissues, such as fibroblasts, connective tissue and lymphocytes. Our observation also showed that the ultrasonic properties were influenced by arrangements of cells and tissue patterns. Conclusions: Our data demonstrate that attenuation and sound speed imaging can provide biomechanical information of the tumor and normal tissues. The results also demonstrate the potential of acoustic microscopy as an auxiliary method for operative detection and localization of cancer affected regions.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2013
Subtitle of host publicationUltrasonic Imaging, Tomography, and Therapy
StatePublished - Jun 5 2013
EventMedical Imaging 2013: Ultrasonic Imaging, Tomography, and Therapy - Lake Buena Vista, FL, United States
Duration: Feb 12 2013Feb 14 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMedical Imaging 2013: Ultrasonic Imaging, Tomography, and Therapy
Country/TerritoryUnited States
CityLake Buena Vista, FL


  • Acoustic attenuation
  • Acoustic microscopy
  • Histology
  • Human breast cancer
  • Pathology
  • Sound speed

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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