Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood

Akankshya Shradhanjali, Susheil Uthamaraj, Dan Dragomir-Daescu, Rajiv Gulati, Gurpreet S. Sandhu, Brandon J. Tefft

Research output: Contribution to journalArticlepeer-review


The endothelium is a dynamic integrated structure that plays an important role in many physiological functions such as angiogenesis, hemostasis, inflammation, and homeostasis. The endothelium also plays an important role in pathophysiologies such as atherosclerosis, hypertension, and diabetes. Endothelial cells form the inner lining of blood and lymphatic vessels and display heterogeneity in structure and function. Various groups have evaluated the functionality of endothelial cells derived from human peripheral blood with a focus on endothelial progenitor cells derived from hematopoietic stem cells or mature blood outgrowth endothelial cells (or endothelial colony-forming cells). These cells provide an autologous resource for therapeutics and disease modeling. Xenogeneic cells may provide an alternative source of therapeutics due to their availability and homogeneity achieved by using genetically similar animals raised in similar conditions. Hence, a robust protocol for the isolation and expansion of highly proliferative blood outgrowth endothelial cells from porcine peripheral blood has been presented. These cells can be used for numerous applications such as cardiovascular tissue engineering, cell therapy, disease modeling, drug screening, studying endothelial cell biology, and in vitro co-cultures to investigate inflammatory and coagulation responses in xenotransplantation.

Original languageEnglish (US)
Article numbere63285
JournalJournal of Visualized Experiments
Issue number179
StatePublished - Jan 2022

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


Dive into the research topics of 'Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood'. Together they form a unique fingerprint.

Cite this