Characterization and quantification of biological micropatterns using cluster SIMS

Li Jung Chen, Sunny S. Shah, Stanislav V. Verkhoturov, Alexander Revzin, Emile A. Schweikert

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Micropatterning is used widely in biosensor development, tissue engineering and basic biology. Creation of biological micropatterns typically involves multiple sequential steps which may lead to cross-contamination and contribute to suboptimal performance of the surface. Therefore, there is a need to develop novel strategies for characterizing location-specific chemical composition of biological micropatterns. In this paper, C60+ time-of-flight secondary ion mass spectrometry (ToF-SIMS) operating in the event-by-event bombardment/detection mode was used for spatially resolved chemical analysis of micropatterned indium tin oxide (ITO) surfaces. Fabrication of the micropatterns involved multiple steps including self-assembly of poly(ethylene glycol)-silane (PEG-silane), patterning of photoresist, treatment with oxygen plasma and adsorption of collagen (I). The ITO surfaces were analyzed with 26-keV C60+ SIMS run in the event-by-event bombardment/detection mode at different steps of the modification process. We were able to evaluate the extent of cross-contamination between different steps and quantify coverage of the immobilized species. The methodology described here provides a novel means for characterizing the composition of biological micropatterns in a quantitative and spatially resolved manner.

Original languageEnglish (US)
Pages (from-to)555-558
Number of pages4
JournalSurface and Interface Analysis
Issue number1-2
StatePublished - Jan 2011


  • C60 SIMS
  • biological micropatterns
  • cluster SIMS
  • collagen micropatterns
  • event-by-event bombardment/detection
  • micropatterns
  • single-impact SIMS

ASJC Scopus subject areas

  • General Chemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry


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