TY - JOUR
T1 - Characterization and chromosomal assignment of yeast artificial chromosomes containing human 3p13-p21-specific sequence tagged sites
AU - Michaelis, Susanne C.
AU - Bardenheuer, Walter
AU - Lux, Andreas
AU - Schramm, Alexander
AU - Gockel, Anja
AU - Siebert, Reiner
AU - Willers, Christoph
AU - Schmidtke, Katja
AU - Todt, Birgit
AU - van der Hout, Annemarie H.
AU - Buys, Charles H.C.M.
AU - Heppell-Parton, Amanda C.
AU - Rabbitts, Pamela H.
AU - Ungar, Sylvia
AU - Smith, David
AU - LePaslier, Denis
AU - Cohen, Daniel
AU - Opalka, Bertram
AU - Schütte, Jochen
N1 - Funding Information:
Susanne Michaelis was supported by a fellowship of the Graduierten-kolleg “Normale und maligne Zellen:’ University of Essen, FRG, Walter Bardenheuer by a fellowship from the Boehringer Ingelheim Fonds, Stuttgart, FRG. FISH analyses were supported by an EMBO fellowship, Heidelberg, FRG (ASI’F 7667). This work was supported by Deutsche Forschungsgemeinschaft, Bonn (SFB 354).
PY - 1995/5
Y1 - 1995/5
N2 - Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, andother neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a chromosome 3p14 microdissection library. STSs were used for isolating yeast artificial chromosome (YAC) clones from the Centre d'Etude du Polymorphisme Humain (CEPH) YAC libraries. Thirty-eight YACs were assembled into a contig approximately 2.5 Mb in size spanning the t(3;8) and t(3;6) translocation breakpoints associated with hereditary renal cell carcinoma and hematologic malignancies, respectively. Chromosomal localization and chimeric status of 126 YACs was analyzed by fluorescence in situ hybridization (FISH). The order of 17 YACs determined by double-color FISH was in agreement with the STS-based arrangement of the YAC-contig.
AB - Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, andother neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a chromosome 3p14 microdissection library. STSs were used for isolating yeast artificial chromosome (YAC) clones from the Centre d'Etude du Polymorphisme Humain (CEPH) YAC libraries. Thirty-eight YACs were assembled into a contig approximately 2.5 Mb in size spanning the t(3;8) and t(3;6) translocation breakpoints associated with hereditary renal cell carcinoma and hematologic malignancies, respectively. Chromosomal localization and chimeric status of 126 YACs was analyzed by fluorescence in situ hybridization (FISH). The order of 17 YACs determined by double-color FISH was in agreement with the STS-based arrangement of the YAC-contig.
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U2 - 10.1016/0165-4608(94)00208-8
DO - 10.1016/0165-4608(94)00208-8
M3 - Article
C2 - 7773951
AN - SCOPUS:0029067485
SN - 0165-4608
VL - 81
SP - 1
EP - 12
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -