TY - JOUR
T1 - Characterisation of hepatitis C virus genotype among blood donors at the regional blood transfusion centre of Ouagadougou, Burkina Faso
AU - Zeba, Moctar Tokèda Abdoul
AU - Sanou, Mahamoudou
AU - Bisseye, Cyrille
AU - Kiba, Alice
AU - Nagalo, Bolni Marius
AU - Djigma, Florencia Wendkuuni
AU - Compaoré, Tegwindé Rebecca
AU - Nebié, Yacouba Koumpingnin
AU - Kienou, Kisito
AU - Sagna, Tani
AU - Pietra, Virginio
AU - Moret, Rémy
AU - Simporé, Jacques
PY - 2014
Y1 - 2014
N2 - Background. Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. Materials and methods. Serum samples were screened for antibodies to HCV using an enzymelinked immunosorbent assay (ARCHITECT-i1000SR- ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). Results. Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4% (95% confidence interval=3.5-5.3) and 1.5% (95% confidence interval=1.0-2.0), respectively. Among HCV RNA carriers, genotyping showed that HCV genotypes 2 and 3 were the most prevalent as they were detected in 18 (56.3%) and 5 (15.6%) individuals, respectively. HCV genotypes 1a and 4 were the least frequent among the blood donors. HCV mixed genotypes 2/3 and 2/4 were also detected among the blood donors. Conclusion. The prevalence of HCV found in this study is lower than previously reported prevalences. Large-scale studies are needed to obtain a better picture of the molecular epidemiology of HCV in Burkina Faso.
AB - Background. Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. Materials and methods. Serum samples were screened for antibodies to HCV using an enzymelinked immunosorbent assay (ARCHITECT-i1000SR- ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). Results. Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4% (95% confidence interval=3.5-5.3) and 1.5% (95% confidence interval=1.0-2.0), respectively. Among HCV RNA carriers, genotyping showed that HCV genotypes 2 and 3 were the most prevalent as they were detected in 18 (56.3%) and 5 (15.6%) individuals, respectively. HCV genotypes 1a and 4 were the least frequent among the blood donors. HCV mixed genotypes 2/3 and 2/4 were also detected among the blood donors. Conclusion. The prevalence of HCV found in this study is lower than previously reported prevalences. Large-scale studies are needed to obtain a better picture of the molecular epidemiology of HCV in Burkina Faso.
KW - Blood donors.
KW - Genotype
KW - HCV
KW - RNA
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U2 - 10.2450/2013.0089-12
DO - 10.2450/2013.0089-12
M3 - Article
C2 - 24599906
AN - SCOPUS:84893924577
SN - 1723-2007
VL - 12
SP - s54-s57
JO - Blood Transfusion
JF - Blood Transfusion
IS - SUPPL.1
ER -