TY - JOUR
T1 - Cerebrospinal Fluid Oligoclonal Bands in the Diagnosis of Multiple Sclerosis
T2 - Isoelectric Focusing with IgG Immunoblotting Compared with High-Resolution Agarose Gel Electrophoresis and Cerebrospinal Fluid IgG Index
AU - Fortini, Alexandre S.
AU - Sanders, Elizabeth L.
AU - Weinshenker, Brian G.
AU - Katzmann, Jerry A.
PY - 2003/11
Y1 - 2003/11
N2 - Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. We compared the diagnostic performance of isoelectric focusing (IEF) combined with IgG immunoblotting to high-resolution agarose gel electrophoresis for the detection of intrathecal synthesis of IgG due to the MS disease process. In 20 patients with definite MS, IEF and high-resolution agarose gel electrophoresis had sensitivities of 90% and 60%, respectively. In the 51 patients with no evidence of MS, the methods had specificities of 94% and 96%, respectively. With a prevalence of 15% in this test population, IEF and high-resolution agarose gel electrophoresis had positive predictive values of 73% and 73% and negative predictive values of 98% and 93%, respectively. The IEF method improves the sensitivity and the negative predictive value of the oligoclonal-banding assay, the IEF gels are easier to interpret, and the IEF assay requires a smaller cerebrospinal fluid volume.
AB - Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. We compared the diagnostic performance of isoelectric focusing (IEF) combined with IgG immunoblotting to high-resolution agarose gel electrophoresis for the detection of intrathecal synthesis of IgG due to the MS disease process. In 20 patients with definite MS, IEF and high-resolution agarose gel electrophoresis had sensitivities of 90% and 60%, respectively. In the 51 patients with no evidence of MS, the methods had specificities of 94% and 96%, respectively. With a prevalence of 15% in this test population, IEF and high-resolution agarose gel electrophoresis had positive predictive values of 73% and 73% and negative predictive values of 98% and 93%, respectively. The IEF method improves the sensitivity and the negative predictive value of the oligoclonal-banding assay, the IEF gels are easier to interpret, and the IEF assay requires a smaller cerebrospinal fluid volume.
KW - Cerebrospinal fluid
KW - Cerebrospinal fluid IgG index
KW - High-resolution agarose gel electrophoresis
KW - Isoelectric focusing
KW - Multiple sclerosis
KW - Oligoclonal bands
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U2 - 10.1309/Y5VFF2UAW0RK5W63
DO - 10.1309/Y5VFF2UAW0RK5W63
M3 - Article
C2 - 14608891
AN - SCOPUS:0242300587
SN - 0002-9173
VL - 120
SP - 672
EP - 675
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 5
ER -