Cerebrospinal fluid calcium homeostasis: evidence for a plasma membrane Ca2+-pump in mammalian choroid plexus

James L. Borke, Ariel J. Caride, Tony L. Yaksh, John T. Penniston, Rajiv Kumar

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


A major unanswered question in central nervous system physiology concerns the mechanism by which cerebrospinal fluid (CSF) Ca2+ homeostasis is maintained in the face of hypo- or hypercalcemia. To address this question, we sought and found a protein of Mr ∼ 140,000 in choroid plexus plasma membranes that forms a phosphorylated intermediate with characteristics of a plasma membrane Ca2+-pump. A choroid plexus plasma membrane protein of this molecular weight also bound to a monoclonal antibody prepared against the human erythrocyte plasma membrane Ca2+-Mg2+ ATPase Ca2+-pump. When this monoclonal antibody was used for immunohistochemical localization, the plasma membrane Ca2+-pump was found primarily in the CSF-facing membranes of choroid plexus cells from rats, cats, and man. The localization of a plasma membrane Ca2+-pump in the CSF-facing membranes of the choroid plexus suggests that the choroid plexus, by mechanisms including this pump, may regulate CSF Ca2+ concentrations.

Original languageEnglish (US)
Pages (from-to)355-360
Number of pages6
JournalBrain Research
Issue number2
StatePublished - Jun 12 1989


  • Ca-Mg ATPase
  • Calcium-pump
  • Calcium-transport
  • Cerebrospinal fluid
  • Choroid plexus
  • Immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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