Caspase-independent autophagic cytotoxicity in etoposide-treated CaSki cervical carcinoma cells

Seung Baek Lee, Seo Yun Tong, Jung Jin Kim, Soo Jong Um, Jong Sup Park

Research output: Contribution to journalArticlepeer-review


We studied the in vitro mechanism of etoposide-induced cell death in cervical cancer cells. Etoposide is cytotoxic to these cells, causing cell death by both apoptosis and autophagy, which has recently been described as a possible mechanism for nonapoptotic cell death. Electron microscopy revealed that autophagosomes/autolysosomes exhibited an autophagic appearance in the presence of etoposide. When autophagy was blocked by inhibitors of autophagy, including 3-methyladenine, both the expression of beclin 1 protein and the antitumor effect of etoposide were suppressed. Benzyloxycarbonyl-Val-Ala-Asp- fluoromethyl ketone, a pan-caspase inhibitor, reduced etoposide-induced cytotoxicity in CaSki cells. Hence, autophagy and apoptosis likely occur concurrently in etoposide-treated cervical cancer cells.

Original languageEnglish (US)
Pages (from-to)713-720
Number of pages8
JournalDNA and Cell Biology
Issue number10
StatePublished - Oct 1 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology


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