TY - JOUR
T1 - Cardiac extracellular matrix proteomics
T2 - Challenges, techniques, and clinical implications
AU - Chang, Chia Wei
AU - Dalgliesh, Ailsa J.
AU - López, Javier E.
AU - Griffiths, Leigh G.
N1 - Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Extracellular matrix (ECM) has emerged as a dynamic tissue component, providing not only structural support, but also functionally participating in a wide range of signaling events during development, injury, and disease remodeling. Investigation of dynamic changes in cardiac ECM proteome is challenging due to the relative insolubility of ECM proteins, which results from their macromolecular nature, extensive post-translational modification (PTM), and tendency to form protein complexes. Finally, the relative abundance of cellular and mitochondrial proteins in cardiac tissue further complicates cardiac ECM proteomic approaches. Recent developments of various techniques to enrich and analyze ECM proteins are playing a major role in overcoming these challenges. Application of cardiac ECM proteomics in disease tissues can further provide spatial and temporal information relevant to disease diagnosis, prognosis, treatment, and engineering of therapeutic candidates for cardiac repair and regeneration.
AB - Extracellular matrix (ECM) has emerged as a dynamic tissue component, providing not only structural support, but also functionally participating in a wide range of signaling events during development, injury, and disease remodeling. Investigation of dynamic changes in cardiac ECM proteome is challenging due to the relative insolubility of ECM proteins, which results from their macromolecular nature, extensive post-translational modification (PTM), and tendency to form protein complexes. Finally, the relative abundance of cellular and mitochondrial proteins in cardiac tissue further complicates cardiac ECM proteomic approaches. Recent developments of various techniques to enrich and analyze ECM proteins are playing a major role in overcoming these challenges. Application of cardiac ECM proteomics in disease tissues can further provide spatial and temporal information relevant to disease diagnosis, prognosis, treatment, and engineering of therapeutic candidates for cardiac repair and regeneration.
KW - Cardiac diseases
KW - Decellularization
KW - Extracellular matrix biomarker
KW - Extracellular matrix remodeling
KW - Extracellular proteomics
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U2 - 10.1002/prca.201500030
DO - 10.1002/prca.201500030
M3 - Review article
C2 - 26200932
AN - SCOPUS:84953345265
SN - 1862-8346
VL - 10
SP - 39
EP - 50
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
IS - 1
ER -