Abstract
Purpose: We investigated whether a clinically used carbonic anhydrase inhibitor (CAIs) can modulate intraocular pressure (IOP) through soluble adenylyl cyclase (sAC) signaling. Methods: IOP was measured 1 h after topical treatment with brinzolamide, a topically applied and clinically used CAIs, using direct cannulation of the anterior chamber in sAC knockout (KO) mice or C57BL/6J mice in the presence or absence of the sAC inhibitor (TDI-10229). Results: Mice treated with the sAC inhibitor TDI-10229 had elevated IOP. CAIs treatment significantly decreased increased intraocular pressure (IOP) in wild-type, sAC KO mice, as well as TDI-10229-treated mice. Conclusions: Inhibiting carbonic anhydrase reduces IOP independently from sAC in mice. Our studies suggest that the signaling cascade by which brinzolamide regulates IOP does not involve sAC.
Original language | English (US) |
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Pages (from-to) | 317-323 |
Number of pages | 7 |
Journal | Journal of Ocular Pharmacology and Therapeutics |
Volume | 39 |
Issue number | 5 |
DOIs | |
State | Published - Jun 1 2023 |
Keywords
- IOP
- cAMP
- carbonic anhydrase
- intraocular pressure
- pharmacological inhibitors
- soluble adenylyl cyclase
ASJC Scopus subject areas
- Ophthalmology
- Pharmacology
- Pharmacology (medical)