TY - JOUR
T1 - c-Myc activation in early coronary lesions in experimental hypercholesterolemia
AU - De Nigris, F.
AU - Lerman, L. O.
AU - Rodriguez-Porcel, M.
AU - De Montis, M. P.
AU - Lerman, A.
AU - Napoli, C.
N1 - Funding Information:
This study was supported in part by Grant ISNIH.99.56980, and HL-03621 and HL-63282 from the National Institutes of Health. We thank Drs. Y. Lee and F. P. D’Armiento for skillful technical support.
PY - 2001
Y1 - 2001
N2 - This study tested the hypothesis that c-Myc activation, an oxidation, sensitive transcription factor, and its binding partner Max occurs in coronary arteries of hypercholesterolemic (HC) pigs, and can be attenuated by chronic antioxidant intervention. Coronary arteries were isolated from normal, HC pigs, or HC supplemented with antioxidant vitamins (HC + vitamins). The expression of the c-Myc/Max complex, and its target genes GADD45 and p53, was studied in nonatherosclerotic, early lesions (LL), positively staining for oil-red-O, in adjacent lesion-prone regions (PL), and in healthy segments (HV). The expression of c-Myc and Max in HC was 2- to 3-fold greater in PL, and 4-fold in LL, compared to normal vessels (P < 0.01). The expression of GADD45 was down-regulated, and of p53 increased, in the same regions. These alterations were attenuated in the HC + vitamins. Thus, c-Myc activation is an early atherosclerosis, in both PL and LL coronary arterial regions, and can be blunted by chronic dietary antioxidant intervention.
AB - This study tested the hypothesis that c-Myc activation, an oxidation, sensitive transcription factor, and its binding partner Max occurs in coronary arteries of hypercholesterolemic (HC) pigs, and can be attenuated by chronic antioxidant intervention. Coronary arteries were isolated from normal, HC pigs, or HC supplemented with antioxidant vitamins (HC + vitamins). The expression of the c-Myc/Max complex, and its target genes GADD45 and p53, was studied in nonatherosclerotic, early lesions (LL), positively staining for oil-red-O, in adjacent lesion-prone regions (PL), and in healthy segments (HV). The expression of c-Myc and Max in HC was 2- to 3-fold greater in PL, and 4-fold in LL, compared to normal vessels (P < 0.01). The expression of GADD45 was down-regulated, and of p53 increased, in the same regions. These alterations were attenuated in the HC + vitamins. Thus, c-Myc activation is an early atherosclerosis, in both PL and LL coronary arterial regions, and can be blunted by chronic dietary antioxidant intervention.
KW - Antioxidants
KW - Atherogenesis
KW - C-Myc
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U2 - 10.1006/bbrc.2001.4431
DO - 10.1006/bbrc.2001.4431
M3 - Article
C2 - 11237752
AN - SCOPUS:0034811562
SN - 0006-291X
VL - 281
SP - 945
EP - 950
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -