Abstract
Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.
Original language | English (US) |
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Pages (from-to) | 229-235 |
Number of pages | 7 |
Journal | Experimental Parasitology |
Volume | 71 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1990 |
Keywords
- Actinomycin D
- Colchicine
- Plasmoduim falciparum
- Pulse labeling
ASJC Scopus subject areas
- Parasitology
- Immunology
- Infectious Diseases