Abstract
Current bone cement systems often demand free radical or metal-related initiators and/or catalysts for the crosslinking process, which may cause serious toxicity to the human body. In addition, the resultant dense scaffolds may have a prolonged degradation time and are difficult for cells to infiltrate and form new tissue. In this study, we developed a porous “click” organic-inorganic nanohybrid (PO-click-ON) cement that crosslinks via metal-free biorthogonal click chemistry and forms porous structures mimicking the native bone tissue via particulate leaching. Strain-promoted click reaction enables fast and efficient crosslinking of polymer chains with the exclusion of any toxic initiator or catalyst. The resulting PO-click-ON implants supported exceptional in vitro stem cell adhesion and osteogenic differentiation with a large portion of stem cells infiltrated deep into the scaffolds. In vivo study using a rat cranial defect model demonstrated that the PO-click-ON system achieved outstanding cell adsorption, neovascularization, and bone formation. The porous click cement developed in this study serves as a promising platform with multifunctionality for bone and other tissue engineering applications.
Original language | English (US) |
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Pages (from-to) | 1585-1597 |
Number of pages | 13 |
Journal | ACS Biomaterials Science and Engineering |
Volume | 9 |
Issue number | 3 |
DOIs | |
State | Published - Mar 13 2023 |
Keywords
- bioinspiration
- bone cements
- click chemistry
- regenerative medicine
- tissue engineering
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering