TY - JOUR
T1 - Binding to gating transduction in nicotinic receptors
T2 - Cys-loop energetically couples to Pre-M1 and M2-M3 regions
AU - Lee, Won Yong
AU - Free, Chris R.
AU - Sine, Steven M.
PY - 2009/3/11
Y1 - 2009/3/11
N2 - The nicotinic acetylcholine receptor (AChR) transduces binding of nerve-released ACh into opening of an intrinsic ion channel, yet the intraprotein interactions behind transduction remain to be fully elucidated. Attention has focused on the region of the AChR in which the β1 -β2 and Cys-loops from the extracellular domain project into a cavity framed by residues preceding the first transmembrane domain (pre-M1) and the linker spanning transmembrane domains M2 and M3. Previous studies identified a principal transduction pathway in which the pre-M1 domain is coupled to the M2-M3 linker through the β1-β2 loop. Here we identify a parallel pathway in which the pre-M1 domain is coupled to the M2-M3 linker through the Cys-loop. Mutagenesis, single-channel kinetic analyses and thermodynamic mutant cycle analyses reveal energetic coupling among αLeu 210 from the pre-M1 domain, αPhe 135 and αPhe 137 from the Cys-loop, and «Leu 273 from the M2-M3 linker. Residues at equivalent positions of non-α-subunits show negligible coupling, indicating these interresidue couplings are specific to residues in the α-subunit. Thus, the extracellular β1-β2 and Cys-loops bridge the pre-M1 domain and M2-M3 linker to transduce agonist binding into channel gating.
AB - The nicotinic acetylcholine receptor (AChR) transduces binding of nerve-released ACh into opening of an intrinsic ion channel, yet the intraprotein interactions behind transduction remain to be fully elucidated. Attention has focused on the region of the AChR in which the β1 -β2 and Cys-loops from the extracellular domain project into a cavity framed by residues preceding the first transmembrane domain (pre-M1) and the linker spanning transmembrane domains M2 and M3. Previous studies identified a principal transduction pathway in which the pre-M1 domain is coupled to the M2-M3 linker through the β1-β2 loop. Here we identify a parallel pathway in which the pre-M1 domain is coupled to the M2-M3 linker through the Cys-loop. Mutagenesis, single-channel kinetic analyses and thermodynamic mutant cycle analyses reveal energetic coupling among αLeu 210 from the pre-M1 domain, αPhe 135 and αPhe 137 from the Cys-loop, and «Leu 273 from the M2-M3 linker. Residues at equivalent positions of non-α-subunits show negligible coupling, indicating these interresidue couplings are specific to residues in the α-subunit. Thus, the extracellular β1-β2 and Cys-loops bridge the pre-M1 domain and M2-M3 linker to transduce agonist binding into channel gating.
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U2 - 10.1523/JNEUROSCI.6185-08.2009
DO - 10.1523/JNEUROSCI.6185-08.2009
M3 - Article
C2 - 19279256
AN - SCOPUS:63849179080
SN - 0270-6474
VL - 29
SP - 3189
EP - 3199
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 10
ER -