Binding of [3H]Benzo(a)pyrene Metabolites to the AKR Mouse Embryo Cell Line Nuclear Proteins

T. H. Zytkovicz, Harold L. Moses, Thomas C. Spelsberg

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Nuclear proteins from AKR-2B mouse embryo cells incubated 24 hr with [3H]benzo(a)pyrene (1 μg/ml; 10 μCi/ml) were purified to remove nucleic acids and characterized by isoelectric focusing and sodium dodecyl sulfate gel electrophoresis on polyacrylamide gels. Analysis of the nuclear proteins by isoelectric focusing revealed a distribution of protein in the pH gradient with two prominent bands of protein, one focusing at pH 5 and the other at pH 11, both of which bound significant amounts of the carcinogen. The acid-soluble proteins from the nuclear preparation were analyzed by isoelectric focusing, and the results also revealed two peaks of protein and radioactivity at pH's of 5 and 11. Further analysis of the acid-soluble fraction on acid urea gels revealed that significant radioactivity was associated with proteins that migrated near but slower than histone H1 which may be the high-mobility group proteins 1 and 2. Radioactivity also comigrated with histones H3 and H2B. Low levels of radioactivity were associated with histone H1 and H4.

Original languageEnglish (US)
Pages (from-to)1608-1614
Number of pages7
JournalCancer research
Issue number5
StatePublished - May 1 1981

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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