TY - JOUR
T1 - Autoantibodies to dense fine speckles in pediatric diseases and controls
AU - Schmeling, Heinrike
AU - Mahler, Michael
AU - Levy, Deborah M.
AU - Moore, Katharine
AU - Stevens, Anne M.
AU - Wick, James
AU - McMillan, Jacob D.
AU - Horneff, Gerd
AU - Assassi, Shervin
AU - Charles, Julio
AU - Salazar, Gloria
AU - Mayes, Maureen D.
AU - Silverman, Earl D.
AU - Klien-Gitelman, Marissa
AU - Lee, Tzelan
AU - Brunner, Hermine I.
AU - Reed, Ann M.
AU - Fritzler, Marvin J.
N1 - Publisher Copyright:
© Copyright 2015 The Journal of Rheumatology. All rights reserved.
PY - 2015/12
Y1 - 2015/12
N2 - Objective. Autoantibodies to the dense fine speckled 70 kDa antigen (DFS70) are reported to be more common in individuals who do not have an antinuclear antibody (ANA)-associated rheumatic disease (AARD) than in patients with AARD. The frequency of anti-DFS70 antibodies has been thoroughly studied in adult but not in pediatric populations. The primary objective of this observational study was to determine the frequency of anti-DFS70 in pediatric AARD and reference cohorts. Methods. Sera from 743 children with AARD and related conditions, and 345 samples from reference cohorts (healthy children and those being investigated for AARD) were studied for anti-DFS70 autoantibodies as measured by a chemiluminescence immunoassay. A de-identified administrative database was used to retrieve demographic, serologic, and clinical data. Results. Anti-DFS70 antibodies were seen in 2.1% of healthy children and in 4.5% of sera from pediatric individuals referred for ANA testing. The frequency of anti-DFS70 was highest in juvenile localized scleroderma (LS; 4/29, 13.8%), juvenile dermatomyositis (JDM; 2/11, 18.2%), childhood systemic lupus erythematosus (cSLE; 19/331, 5.7%), diffuse cutaneous systemic sclerosis (1/22, 4.5%), celiac disease (2/49, 4.1%), and juvenile idiopathic arthritis (JIA; 5/202, 2.5%). Of note, anti-DFS70 antibodies were observed in 3/26 children (11.5%) with uveitis and JIA-associated uveitis. Conclusion. The frequency of anti-DFS70 autoantibodies in healthy pediatric subjects is within the lower range of that reported in adults. Anti-DFS70 antibodies can be found in childhood SSc and cSLE, but has a remarkably high frequency in children with LS, JDM, and uveitis.
AB - Objective. Autoantibodies to the dense fine speckled 70 kDa antigen (DFS70) are reported to be more common in individuals who do not have an antinuclear antibody (ANA)-associated rheumatic disease (AARD) than in patients with AARD. The frequency of anti-DFS70 antibodies has been thoroughly studied in adult but not in pediatric populations. The primary objective of this observational study was to determine the frequency of anti-DFS70 in pediatric AARD and reference cohorts. Methods. Sera from 743 children with AARD and related conditions, and 345 samples from reference cohorts (healthy children and those being investigated for AARD) were studied for anti-DFS70 autoantibodies as measured by a chemiluminescence immunoassay. A de-identified administrative database was used to retrieve demographic, serologic, and clinical data. Results. Anti-DFS70 antibodies were seen in 2.1% of healthy children and in 4.5% of sera from pediatric individuals referred for ANA testing. The frequency of anti-DFS70 was highest in juvenile localized scleroderma (LS; 4/29, 13.8%), juvenile dermatomyositis (JDM; 2/11, 18.2%), childhood systemic lupus erythematosus (cSLE; 19/331, 5.7%), diffuse cutaneous systemic sclerosis (1/22, 4.5%), celiac disease (2/49, 4.1%), and juvenile idiopathic arthritis (JIA; 5/202, 2.5%). Of note, anti-DFS70 antibodies were observed in 3/26 children (11.5%) with uveitis and JIA-associated uveitis. Conclusion. The frequency of anti-DFS70 autoantibodies in healthy pediatric subjects is within the lower range of that reported in adults. Anti-DFS70 antibodies can be found in childhood SSc and cSLE, but has a remarkably high frequency in children with LS, JDM, and uveitis.
KW - Antinuclear antibodies
KW - Autoantibodies
KW - Autoimmune diseases
KW - Dense fine speckles
KW - Pediatrics
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U2 - 10.3899/jrheum.150567
DO - 10.3899/jrheum.150567
M3 - Article
C2 - 26472409
AN - SCOPUS:84948845058
SN - 0315-162X
VL - 42
SP - 2419
EP - 2426
JO - Journal of Rheumatology
JF - Journal of Rheumatology
IS - 12
ER -