TY - JOUR
T1 - Attenuation by butalbital of capsaicin-induced C-fos-like immunoreactivity in trigeminal nucleus caudalis
AU - Cutrer, F. Michael
AU - Mitsikostas, Dimos D.
AU - Ayata, Gamze
AU - Sanchez Del Rio, Margarita
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1999
Y1 - 1999
N2 - We examined the effects of butalbital (30, 100, and 1000 μg/kg) on the number of cells expressing c-fos-like immunoreactivity (c-fos-LI), a marker of neuronal activation, within lamina I, II(o) of the trigeminal nucleus caudalis and the nucleus of the solitary tract 2 hours after the intracisternal injection of capsaicin (0.1 mL; 15.25 mg/mL) or vehicle in urethane-anesthetized guinea pigs (N=45). Robust c-fos-LI was observed within nuclei of cells in the trigeminal nucleus caudalis after capsaicin (329 ± 35). Butalbital dose-dependently reduced the number of labeled cells to a maximum of 66% (1000 μg/kg intraperitoneally [IP], P<.01) in lamina I, II(o) but not within area postrema, medial reticular nucleus, or the nucleus of the solitary tract. Pretreatment with bicuculline (30 μg/kg IP) blocked the effect of butalbital, thereby suggesting the importance of the GABA(A) receptor to activation involved in the transmission of nociceptive information. Our studies suggest the possibility that GABA(A) receptors might provide an important therapeutic target in migraine and related headache disorders.
AB - We examined the effects of butalbital (30, 100, and 1000 μg/kg) on the number of cells expressing c-fos-like immunoreactivity (c-fos-LI), a marker of neuronal activation, within lamina I, II(o) of the trigeminal nucleus caudalis and the nucleus of the solitary tract 2 hours after the intracisternal injection of capsaicin (0.1 mL; 15.25 mg/mL) or vehicle in urethane-anesthetized guinea pigs (N=45). Robust c-fos-LI was observed within nuclei of cells in the trigeminal nucleus caudalis after capsaicin (329 ± 35). Butalbital dose-dependently reduced the number of labeled cells to a maximum of 66% (1000 μg/kg intraperitoneally [IP], P<.01) in lamina I, II(o) but not within area postrema, medial reticular nucleus, or the nucleus of the solitary tract. Pretreatment with bicuculline (30 μg/kg IP) blocked the effect of butalbital, thereby suggesting the importance of the GABA(A) receptor to activation involved in the transmission of nociceptive information. Our studies suggest the possibility that GABA(A) receptors might provide an important therapeutic target in migraine and related headache disorders.
KW - Capsaicin
KW - Guinea pig
KW - Immunohistochemistry
KW - Intracisternal
KW - Trigeminal nucleus caudalis
KW - c- fos
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U2 - 10.1046/j.1526-4610.1999.3910697.x
DO - 10.1046/j.1526-4610.1999.3910697.x
M3 - Article
C2 - 11279945
AN - SCOPUS:0033394575
SN - 0017-8748
VL - 39
SP - 697
EP - 704
JO - Headache
JF - Headache
IS - 10
ER -