Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH)

Gopanandan Parthasarathy; Harmeet Malhi

doi:10.1007/978-1-0716-2128-8_19

Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH)

Gopanandan Parthasarathy, Harmeet Malhi

Gastroenterology and Hepatology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Hepatocyte lipotoxicity is a hallmark of nonalcoholic steatohepatitis (NASH), and lipid induced liver injury occurs, in part, via activation of endoplasmic reticulum (ER) stress. Consequently, the unfolded protein response (UPR) is initiated, driven by three key ER transmembrane proteins, resulting in downstream responses that are dynamic and interconnected. Thus, careful interrogation of these pathways is required to investigate the complex role of ER stress in NASH. Herein, we describe different mechanisms of, and in vitro assays for assessment of lipotoxic ER stress in mouse hepatocytes.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	243-254
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-0716-2128-8_19
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2455
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Apoptosis
C/EBP homologous binding protein
Free fatty acid
Palmitic acid
Unfolded protein response
X-box binding protein 1

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-0716-2128-8_19

Cite this

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abstract = "Hepatocyte lipotoxicity is a hallmark of nonalcoholic steatohepatitis (NASH), and lipid induced liver injury occurs, in part, via activation of endoplasmic reticulum (ER) stress. Consequently, the unfolded protein response (UPR) is initiated, driven by three key ER transmembrane proteins, resulting in downstream responses that are dynamic and interconnected. Thus, careful interrogation of these pathways is required to investigate the complex role of ER stress in NASH. Herein, we describe different mechanisms of, and in vitro assays for assessment of lipotoxic ER stress in mouse hepatocytes.",

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AB - Hepatocyte lipotoxicity is a hallmark of nonalcoholic steatohepatitis (NASH), and lipid induced liver injury occurs, in part, via activation of endoplasmic reticulum (ER) stress. Consequently, the unfolded protein response (UPR) is initiated, driven by three key ER transmembrane proteins, resulting in downstream responses that are dynamic and interconnected. Thus, careful interrogation of these pathways is required to investigate the complex role of ER stress in NASH. Herein, we describe different mechanisms of, and in vitro assays for assessment of lipotoxic ER stress in mouse hepatocytes.

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KW - Free fatty acid

KW - Palmitic acid

KW - Unfolded protein response

KW - X-box binding protein 1

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ER -

Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH)

Abstract

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Keywords

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