Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium

Martin Rodriguez-Porcel; Olivier Gheysens; Ramasamy Paulmurugan; Ian Y. Chen; Karen M. Peterson; Jürgen K. Willmann; Joseph C. Wu; Xiangyang Zhu; Lilach O. Lerman; Sanjiv S. Gambhir

doi:10.1007/s11307-009-0274-4

Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium

Martin Rodriguez-Porcel, Olivier Gheysens, Ramasamy Paulmurugan, Ian Y. Chen, Karen M. Peterson, Jürgen K. Willmann, Joseph C. Wu, Xiangyang Zhu, Lilach O. Lerman, Sanjiv S. Gambhir

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.

Original language	English (US)
Pages (from-to)	325-334
Number of pages	10
Journal	Molecular Imaging and Biology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1007/s11307-009-0274-4
State	Published - Jun 2010

Keywords

Antioxidants
Bioluminescence
Firefly luciferase
Molecular imaging
Myoblasts
Myocardium
Oxidative stress
Stem cell

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

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10.1007/s11307-009-0274-4

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title = "Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium",

abstract = "Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.",

keywords = "Antioxidants, Bioluminescence, Firefly luciferase, Molecular imaging, Myoblasts, Myocardium, Oxidative stress, Stem cell",

author = "Martin Rodriguez-Porcel and Olivier Gheysens and Ramasamy Paulmurugan and Chen, {Ian Y.} and Peterson, {Karen M.} and Willmann, {J{\"u}rgen K.} and Wu, {Joseph C.} and Xiangyang Zhu and Lerman, {Lilach O.} and Gambhir, {Sanjiv S.}",

note = "Funding Information: Funding. This work was supported in part by National Health Lung Blood Institute R01 HL078632 (SSG), National Cancer Institute ICMIC CA114747 P50 (SSG), National Institutes of Health K99-R00 HL88048 (MR-P), and the Mayo Clinical Scholarship Program, Mayo Clinic College of Medicine, Rochester, Minnesota (MR-P).",

year = "2010",

month = jun,

doi = "10.1007/s11307-009-0274-4",

language = "English (US)",

volume = "12",

pages = "325--334",

journal = "Molecular Imaging and Biology",

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T1 - Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium

AU - Rodriguez-Porcel, Martin

AU - Gheysens, Olivier

AU - Paulmurugan, Ramasamy

AU - Chen, Ian Y.

AU - Peterson, Karen M.

AU - Willmann, Jürgen K.

AU - Wu, Joseph C.

AU - Zhu, Xiangyang

AU - Lerman, Lilach O.

AU - Gambhir, Sanjiv S.

N1 - Funding Information: Funding. This work was supported in part by National Health Lung Blood Institute R01 HL078632 (SSG), National Cancer Institute ICMIC CA114747 P50 (SSG), National Institutes of Health K99-R00 HL88048 (MR-P), and the Mayo Clinical Scholarship Program, Mayo Clinic College of Medicine, Rochester, Minnesota (MR-P).

PY - 2010/6

Y1 - 2010/6

N2 - Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.

AB - Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.

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KW - Bioluminescence

KW - Firefly luciferase

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KW - Myoblasts

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KW - Oxidative stress

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Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium

Abstract

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ASJC Scopus subject areas

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