TY - JOUR
T1 - Antibodies to the cytoplasmic domain of the MUC1 mucin show conservation throughout mammals
AU - Pemberton, Lucy
AU - Taylor-Papadimitriou, Joyce
AU - Gendler, Sandra J.
PY - 1992/5/29
Y1 - 1992/5/29
N2 - An antiserum against the carboxy-terminal seventeen amino acids of the human MUC1 mucin has been raised and extensively characterized. This antiserum, CT1, immunoprecipitates two high molecular weight polymorphic bands (>200 kDa) from a metabolically labelled breast cancer cell line corresponding to the two alleles which have previously been shown to contain different numbers of a twenty amino acid repeat. The CT1 antiserum reacted with tissues from many mammalian species and immunoprecipitated large polymorphic proteins, suggesting that the cytoplasmic portion of the molecule is well conserved. The cell and tissue distribution of Muc-1 mucin in the mouse has been studied by immunocytochemistry. This protein is abundant at the apical surfaces of epithelial tissues and is found expressed in the stomach, kidney, mammary gland, pancreas, salivary gland, lung, trachea, uterus, cervix and vagina.
AB - An antiserum against the carboxy-terminal seventeen amino acids of the human MUC1 mucin has been raised and extensively characterized. This antiserum, CT1, immunoprecipitates two high molecular weight polymorphic bands (>200 kDa) from a metabolically labelled breast cancer cell line corresponding to the two alleles which have previously been shown to contain different numbers of a twenty amino acid repeat. The CT1 antiserum reacted with tissues from many mammalian species and immunoprecipitated large polymorphic proteins, suggesting that the cytoplasmic portion of the molecule is well conserved. The cell and tissue distribution of Muc-1 mucin in the mouse has been studied by immunocytochemistry. This protein is abundant at the apical surfaces of epithelial tissues and is found expressed in the stomach, kidney, mammary gland, pancreas, salivary gland, lung, trachea, uterus, cervix and vagina.
UR - http://www.scopus.com/inward/record.url?scp=0026716584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026716584&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)80971-4
DO - 10.1016/S0006-291X(05)80971-4
M3 - Article
C2 - 1599454
AN - SCOPUS:0026716584
SN - 0006-291X
VL - 185
SP - 167
EP - 175
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -